干旱区生态环境知识资源中心(Arid): Genetic polymorphisms of Plasmodium falciparum isolates from Melka-Werer, North East Ethiopia based on the merozoite surface protein-2 (msp-2) gene as a molecular marker

Arid

DOI	10.1186/s12936-021-03625-1
	Genetic polymorphisms of Plasmodium falciparum isolates from Melka-Werer, North East Ethiopia based on the merozoite surface protein-2 (msp-2) gene as a molecular marker
	Mohammed, Hussein; Assefa, Ashenafi; Chernet, Melkie; Wuletaw, Yonas; Commons, Robert J.
通讯作者	Mohammed, H (corresponding author), Ethiopian Publ Hlth Inst, Parasit Zoonot Dis Res Directorate, Malaria Neglected Trop Dis Res Team, Addis Ababa, Ethiopia.
来源期刊	MALARIA JOURNAL
EISSN	1475-2875
出版年	2021
卷号	20 期号:1
英文摘要	Background The characterization of parasite populations circulating in malaria endemic areas is necessary to evaluate the success of ongoing interventions and malaria control strategies. This study was designed to investigate the genetic diversity of Plasmodium falciparum isolates from the semi-arid area in North East Ethiopia, using the highly polymorphic merozoite surface protein-2 (msp2) gene as a molecular marker. Methods Dried blood spot isolates were collected from patients with P. falciparum infection between September 2014 and January 2015 from Melka-Werer, North East Ethiopia. Parasite DNA was extracted and genotyped using allele-specific nested polymerase chain reactions for msp2. Results 52 isolates were collected with msp2 identified in 41 (78.8%) isolates. Allele typing of the msp2 gene detected the 3D7/IC allelic family in 54% and FC27 allelic family in 46%. A total of 14 different msp2 genotypes were detected including 6 belonging to the 3D7/IC family and 8 to the FC27 family. Forty percent of isolates had multiple genotypes and the overall mean multiplicity of infections (MOI) was 1.2 (95%CI 0.96-1.42). The heterozygosity index was 0.50 for the msp2 locus. There was no difference in MOI between age groups. A negative correlation between parasite density and multiplicity of infection was found (p=0.02). Conclusion Plasmodium falciparum isolates from the semi-arid area of North East Ethiopia are mainly monoclonal with low MOI and limited genetic diversity in the study population.
英文关键词	Ethiopia Genotyping Polymorphism Merozoite surface protein 2 Multiplicity of infection
类型	Article
语种	英语
开放获取类型	gold, Green Submitted, Green Published
收录类别	SCI-E
WOS记录号	WOS:000619700800001
WOS类目	Infectious Diseases ; Parasitology ; Tropical Medicine
WOS研究方向	Infectious Diseases ; Parasitology ; Tropical Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348057
作者单位	[Mohammed, Hussein; Assefa, Ashenafi; Chernet, Melkie; Wuletaw, Yonas] Ethiopian Publ Hlth Inst, Parasit Zoonot Dis Res Directorate, Malaria Neglected Trop Dis Res Team, Addis Ababa, Ethiopia; [Commons, Robert J.] Charles Darwin Univ, Menzies Sch Hlth Res, Global Hlth Div, Darwin, NT, Australia; [Commons, Robert J.] Internal Med Serv, Ballarat Hlth Serv, Ballarat, Vic, Australia
推荐引用方式 GB/T 7714	Mohammed, Hussein,Assefa, Ashenafi,Chernet, Melkie,et al. Genetic polymorphisms of Plasmodium falciparum isolates from Melka-Werer, North East Ethiopia based on the merozoite surface protein-2 (msp-2) gene as a molecular marker[J],2021,20(1).
APA	Mohammed, Hussein,Assefa, Ashenafi,Chernet, Melkie,Wuletaw, Yonas,&Commons, Robert J..(2021).Genetic polymorphisms of Plasmodium falciparum isolates from Melka-Werer, North East Ethiopia based on the merozoite surface protein-2 (msp-2) gene as a molecular marker.MALARIA JOURNAL,20(1).
MLA	Mohammed, Hussein,et al."Genetic polymorphisms of Plasmodium falciparum isolates from Melka-Werer, North East Ethiopia based on the merozoite surface protein-2 (msp-2) gene as a molecular marker".MALARIA JOURNAL 20.1(2021).