干旱区生态环境知识资源中心(Arid): Differential responses of the antennal proteome of male and female migratory locusts to infection by a fungal pathogen

Arid

DOI	10.1016/j.jprot.2020.104050
	Differential responses of the antennal proteome of male and female migratory locusts to infection by a fungal pathogen
	Zheng, Renwen; Xia, Yuxian; Keyhani, Nemat O.
通讯作者	Xia, YX (corresponding author), Chongqing Univ, Chongqing Engn Res Ctr Fungal Insecticides, Sch Life Sci, Chongqing 400030, Peoples R China. ; Keyhani, NO (corresponding author), Univ Florida, Inst Food & Agr Sci, Dept Microbiol & Cell Sci, Bldg 981,Museum Rd, Gainesville, FL 32611 USA.
来源期刊	JOURNAL OF PROTEOMICS
ISSN	1874-3919
EISSN	1876-7737
出版年	2021
卷号	232
英文摘要	The narrow host range entomopathogenic fungus, Metarhizium acridum, is an environmentally friendly acridid specific pathogen used for locust control. The locust is capable of responding within hours of infection, however, little is known concerning how the locust detects the pathogen. Here, we have identified 3213 proteins in the infected antennal proteome of the migratory locust, Locusta migratoria. iTRAQ comparative analyses of antennal proteomes identified 194 differentially abundant proteins (DAPs) between uninfected and infected males, 218 DAPs between uninfected and infected females, and 240 DAPs between infected males and infected females. In relation to olfaction, a total of 29 chemosensory proteins (CSPs), 9 odorant binding proteins (OBPs), 31 odorant receptors (ORs), and 8 ionotropic receptors (IRs) were differentially abundant after M. acridum infection, with a subset of 12 proteins found in both infected male and female antennae not present in uninfected individuals. The time course of the gene expression profiles of olfaction related DAPs were investigated by quantitative real-time PCR (qRT-PCR). Our data indicate significant changes in the antennal proteomes of male and female locusts in response to a microbial pathogen, highlighting the potential participation of olfactory processes in pathogen detection and response. Biological significance: The ability of an organism to detect microbial pathogens is essential for mounting a response to mitigate the spread of the infection. Using iTRAQ-based proteomic analyses changes in the protein repertoire of the antennae of male and female locusts in response to infection by a host-specific pathogen were determined. These data show proteomic alterations that are also sex-specific, identifying members of olfactory pathways that are modified in response to infection. Our data identify antennal and related olfactory proteins that are candidates for mediating host detection of pathogens, and that may contribute to subsequent behavioral and/or immune responses of the host to the infection challenge.
英文关键词	Locust Antennae Insect pathogenic fungus Metarhizium acridum Olfaction Sex specific variation
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000606630600002
WOS关键词	ODORANT-BINDING PROTEINS ; ZOOPHTHORA RADICANS INFECTION ; ENTOMOPATHOGENIC FUNGI ; MINIMUM INFORMATION ; DESERT LOCUST ; PHEROMONE ; HOST ; RECEPTORS ; VIRULENCE ; GENOMICS
WOS类目	Biochemical Research Methods
WOS研究方向	Biochemistry & Molecular Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348034
作者单位	[Zheng, Renwen; Xia, Yuxian] Chongqing Univ, Chongqing Engn Res Ctr Fungal Insecticides, Sch Life Sci, Chongqing 400030, Peoples R China; [Keyhani, Nemat O.] Univ Florida, Inst Food & Agr Sci, Dept Microbiol & Cell Sci, Bldg 981,Museum Rd, Gainesville, FL 32611 USA
推荐引用方式 GB/T 7714	Zheng, Renwen,Xia, Yuxian,Keyhani, Nemat O.. Differential responses of the antennal proteome of male and female migratory locusts to infection by a fungal pathogen[J],2021,232.
APA	Zheng, Renwen,Xia, Yuxian,&Keyhani, Nemat O..(2021).Differential responses of the antennal proteome of male and female migratory locusts to infection by a fungal pathogen.JOURNAL OF PROTEOMICS,232.
MLA	Zheng, Renwen,et al."Differential responses of the antennal proteome of male and female migratory locusts to infection by a fungal pathogen".JOURNAL OF PROTEOMICS 232(2021).