干旱区生态环境知识资源中心(Arid): Simultaneous determination of fructose, glucose and sucrose by solid phase extraction-liquid chromatography-tandem mass spectrometry and its application to source and adulteration analysis of sucrose in tea

Arid

DOI	10.1016/j.jfca.2020.103730
	Simultaneous determination of fructose, glucose and sucrose by solid phase extraction-liquid chromatography-tandem mass spectrometry and its application to source and adulteration analysis of sucrose in tea
	Wang, Hui; Hu, Lili; Zhou, Peng; Ouyang, Li; Chen, Bo; Li, Ying; Chen, Yaya; Zhang, Yan; Zhou, Jinsha
通讯作者	Wang, H (corresponding author), Changsha Inst Food & Drug Control, Changsha 410013, Peoples R China. ; Wang, H (corresponding author), Natl Alcohol Prod Qual Supervis & Inspect Ctr, Changsha 410013, Peoples R China. ; Chen, B (corresponding author), Hunan Normal Univ, Coll Chem & Chem Engn, Key Lab Phytochem R&D Hunan Prov, Minist Educ, Changsha 410081, Peoples R China. ; Chen, B (corresponding author), Hunan Normal Univ, Coll Chem & Chem Engn, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ, Changsha 410081, Peoples R China.
来源期刊	JOURNAL OF FOOD COMPOSITION AND ANALYSIS
ISSN	0889-1575
EISSN	1096-0481
出版年	2021
卷号	96
英文摘要	A solid phase extraction-liquid chromatography-tandem mass spectrometry (SPE-LC-MS/MS) method was developed for simultaneous determination of fructose, glucose and sucrose in tea. Tea samples were ultrasonically extracted with ultrapure water and cleaned up by Oasis PRIME HLB SPE cartridge. The separation of the analytes was performed on XBridge BEH Amide column (2.1 x 150 mm, 5 mu m) with isocratic elution. Detection of the target compounds were carried out under the multiple reaction monitoring (MRM) mode with negative electrospray ionization (ESI-). The qualitative screening was achieved with retention time and qualitative ion, then confirmation analysis with peak area and quantitative ion. The limits of detection (LODs, S/N >= 3) of fructose, glucose and sucrose were 0.02 mg/L, 0.03 mg/L and 0.08 mg/L, respectively. The average recoveries varied from 92.1 %-108.5 % for three levels. Linearity was obtained with the correlation coefficients greater than 0.992 for three target compounds. The established method was rapid, simple and accurate which was suitable for batch determination of fructose, glucose and sucrose in tea. A total of 213 tea samples from 7 countries were analysed and the results statistically analyzed using MATLAB software. Moreover, source and adulteration analysis of sucrose in tea was carried out. Sucrose in tea is mainly derived from photosynthesis during growth, hydrolysis of polysaccharides during the processing and artificial adulteration, once the sucrose content in tea exceeds 24.3 g/kg, it may involve artificial adulteration.
英文关键词	Solid phase extraction Liquid chromatography-tandem mass spectrometry Sucrose Source analysis Adulteration analysis Tea
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000607029700009
WOS关键词	GREEN TEA ; LC-MS/MS ; QUANTIFICATION ; POLYPHENOLS ; SINENSIS ; QUALITY ; ETHANOL
WOS类目	Chemistry, Applied ; Food Science & Technology
WOS研究方向	Chemistry ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348009
作者单位	[Wang, Hui; Hu, Lili; Zhou, Peng; Ouyang, Li; Li, Ying; Chen, Yaya; Zhou, Jinsha] Changsha Inst Food & Drug Control, Changsha 410013, Peoples R China; [Wang, Hui; Hu, Lili; Zhou, Peng; Ouyang, Li; Li, Ying; Chen, Yaya; Zhou, Jinsha] Natl Alcohol Prod Qual Supervis & Inspect Ctr, Changsha 410013, Peoples R China; [Chen, Bo] Hunan Normal Univ, Coll Chem & Chem Engn, Key Lab Phytochem R&D Hunan Prov, Minist Educ, Changsha 410081, Peoples R China; [Chen, Bo] Hunan Normal Univ, Coll Chem & Chem Engn, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ, Changsha 410081, Peoples R China; [Zhang, Yan] Yangtze Univ, Coll Life Sci, Jingzhou 434025, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hui,Hu, Lili,Zhou, Peng,et al. Simultaneous determination of fructose, glucose and sucrose by solid phase extraction-liquid chromatography-tandem mass spectrometry and its application to source and adulteration analysis of sucrose in tea[J],2021,96.
APA	Wang, Hui.,Hu, Lili.,Zhou, Peng.,Ouyang, Li.,Chen, Bo.,...&Zhou, Jinsha.(2021).Simultaneous determination of fructose, glucose and sucrose by solid phase extraction-liquid chromatography-tandem mass spectrometry and its application to source and adulteration analysis of sucrose in tea.JOURNAL OF FOOD COMPOSITION AND ANALYSIS,96.
MLA	Wang, Hui,et al."Simultaneous determination of fructose, glucose and sucrose by solid phase extraction-liquid chromatography-tandem mass spectrometry and its application to source and adulteration analysis of sucrose in tea".JOURNAL OF FOOD COMPOSITION AND ANALYSIS 96(2021).