干旱区生态环境知识资源中心(Arid): Duration and degree of diet-induced metabolic acidosis prepartum alter tissue responses to insulin in dairy cows

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DOI	10.3168/jds.2020-18787
	Duration and degree of diet-induced metabolic acidosis prepartum alter tissue responses to insulin in dairy cows
	Vieira-Neto, A.; Zimpel, R.; Lopes, F. R., Jr.; Scheffler, T. L.; Block, E.; Thatcher, W. W.; Santos, J. E. P.
通讯作者	Santos, JEP (corresponding author), Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. ; Santos, JEP (corresponding author), Univ Florida, DH Barron Reprod & Perinatal Biol Res Program, Gainesville, FL 32611 USA.
来源期刊	JOURNAL OF DAIRY SCIENCE
ISSN	0022-0302
EISSN	1525-3198
出版年	2021
卷号	104 期号:2 页码:1660-1679
英文摘要	The objective of this experiment was to determine the effects of altering the dietary cation-anion difference (DCAD) fed for the last 21 or 42 d of gestation on glucose metabolism and tissue insulin responsiveness. Ninety parous Holstein cows at 232 d of gestation were assigned randomly to dietary treatments with 2 levels of DCAD (-70 or -180 mEq/kg) fed for 2 durations (short: the last 21 d of gestation; long: the last 42 d of gestation). For the short treatments, a diet with +110 mEq/kg was fed from 232 to 254 d of gestation. Intravenous glucose tolerance tests (IVGTT) were performed at either 250 or 270 d of gestation by infusing 0.25 g of dextrose/kg of body weight within 1 min. The following day, cows underwent an insulin challenge (IC) and received 0.1 IU of insulin/kg of body weight intravenously. Blood was sampled at min -15, -5, arid 0 to establish a baseline and from 5 to 180 min relative to infusions; plasma concentrations of glucose, insulin, and fatty acids were determined, and the respective areas under the curves (AUC) were calculated. Liver was sampled after the IVGTT, and adipose tissue was sampled after the IVGTT and IC for quantification of mRNA expression and protein abundance. Reducing the DCAD altered acid base balance compatible with a compensated metabolic acidosis. At 250 d, reducing the DCAD increased the AUC for glucose and reduced that of insulin following the IVGTT, whereas during the IC, clearance rate decreased and time to half-life of insulin increased with reducing DCAD, resulting in a tendency to a larger AUC for fatty acids. At 270 d, quantitative insulin sensitivity check index and the revised quantitative insulin sensitivity check index were smaller in cows fed the acidogenic diets for the last 42 d of gestation compared with the last 21 d of gestation, thereby suggesting reduced insulin sensitivity. In addition, cows fed for the long duration tended to have greater AUC for glucose but smaller AUC for insulin following an IVGTT than those fed for the short duration, thereby suggesting reduced insulin release and glucose disposal. Treatments did not affect hepatic mRNA expression of G6PC, PCK1, PCK2, and PC or adipose tissue mRNA expression of ATGL, ACC, B2AR, HSL, and PLIN1. On the other hand, for proteins, reducing the DCAD linearly reduced abundance of rabbit anti-mouse protein kinase B (AKT) and tended to reduce rabbit anti-human phosphorylated (Ser-9) glycogen synthase kinase-3 (pGSK) arid the pGSK:rabbit anti-human glycogen synthase kinase-3 R (GSK) ratio in hepatic tissue, whereas a linear increase in rabbit anti-human hormone-sensitive lipase (HSL) and rabbit anti-mouse phosphorylated (Ser-660) hormone-sensitive lipase (pHSL) in adipose tissue was observed after the IVGTT at 250 d. Moreover, reducing the DCAD resulted in a linear reduction of AKT and tended to reduce rabbit anti-human acetyl-CoA carboxylase (ACC) but increased pHSL linearly in adipose tissue after an IC at 250 d. Cows fed acidogenic diets for a short duration tended to have less pHSL in adipose tissue than those fed for a long duration after an IVGTT at 270 d. Associations were observed between blood pH and mRNA and protein abundance in hepatic and adipose tissues. Diet-induced metabolic acidosis altered insulin release and insulin signaling, resulting in a shift in adipose tissue metabolism that would favor lipolysis over lipogenesis.
英文关键词	acidogenic diet dairy cow glucose insulin
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000608140300035
WOS关键词	GLUCOSE-TOLERANCE TESTS ; CATION-ANION DIFFERENCE ; SKELETAL-MUSCLE ; PHOSPHORYLATION ; GROWTH ; HYPOCALCEMIA ; PERFORMANCE ; HOMEOSTASIS ; RESISTANCE ; LACTATION
WOS类目	Agriculture, Dairy & Animal Science ; Food Science & Technology
WOS研究方向	Agriculture ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/347998
作者单位	[Vieira-Neto, A.; Zimpel, R.; Lopes, F. R., Jr.; Scheffler, T. L.; Thatcher, W. W.; Santos, J. E. P.] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA; [Vieira-Neto, A.; Zimpel, R.; Thatcher, W. W.; Santos, J. E. P.] Univ Florida, DH Barron Reprod & Perinatal Biol Res Program, Gainesville, FL 32611 USA; [Block, E.] Arm & Hammer Anim Nutr, Princeton, NJ 08543 USA
推荐引用方式 GB/T 7714	Vieira-Neto, A.,Zimpel, R.,Lopes, F. R., Jr.,et al. Duration and degree of diet-induced metabolic acidosis prepartum alter tissue responses to insulin in dairy cows[J],2021,104(2):1660-1679.
APA	Vieira-Neto, A..,Zimpel, R..,Lopes, F. R., Jr..,Scheffler, T. L..,Block, E..,...&Santos, J. E. P..(2021).Duration and degree of diet-induced metabolic acidosis prepartum alter tissue responses to insulin in dairy cows.JOURNAL OF DAIRY SCIENCE,104(2),1660-1679.
MLA	Vieira-Neto, A.,et al."Duration and degree of diet-induced metabolic acidosis prepartum alter tissue responses to insulin in dairy cows".JOURNAL OF DAIRY SCIENCE 104.2(2021):1660-1679.