干旱区生态环境知识资源中心(Arid): Exploiting a perchlorate-tolerant desert cyanobacterium to support bacterial growth for in situ resource utilization on Mars

Arid

DOI	10.1017/S1473550420000300
	Exploiting a perchlorate-tolerant desert cyanobacterium to support bacterial growth for in situ resource utilization on Mars
	Billi, Daniela; Fernandez, Beatriz Gallego; Fagliarone, Claudia; Chiavarini, Salvatore; Rothschild, Lynn Justine
通讯作者	Billi, D (corresponding author), Univ Roma Tor Vergata, Dept Biol, Lab Astrobiol & Mol Biol Cyanobacteria, Rome, Italy.
来源期刊	INTERNATIONAL JOURNAL OF ASTROBIOLOGY
ISSN	1473-5504
EISSN	1475-3006
出版年	2021
卷号	20 期号:1 页码:29-35
英文摘要	The presence of perchlorate in the Martian soil may limit in-situ resource utilization (ISRU) technologies to support human outposts. In order to exploit the desiccation, radiation-tolerant cyanobacterium Chroococcidopsis in Biological Life Support Systems based on ISRU, we investigated the perchlorate tolerance of Chroococcidopsis sp. CCMEE 029 and its derivative CCMEE 029 P-MRS. This strain was obtained from dried cells mixed with Martian regolith simulant and exposed to Mars-like conditions during the BIOMEX space experiment. After a 55-day exposure of up to 200 mM perchlorate ions, a tolerance threshold value of 100 mM perchlorate ions was identified for both Chroococcidopsis strains. After 40-day incubation, a Mars-relevant perchlorate concentration of 2.4 mM perchlorate ions, provided as a 60 and 40% mixture of Mg- and Ca-perchlorate, had no negative effect on the growth rate of the two strains. A proof-of-concept experiment was conducted using Chroococcidopsis lysate in ISRU technologies to feed a heterotrophic bacterium, i.e. an Escherichia coli strain capable of metabolizing sucrose. The sucrose content was fivefold increased in Chroococcidopsis cells through air-drying and the yielded lysate successfully supported the bacterial growth. This suggested that Chroococcidopsis is a suitable candidate for ISRU technologies to support heterotrophic BLSS components in a Mars-relevant perchlorate environment that would prove challenging to many other cyanobacteria, allowing a 'live off the land' approach on Mars.
英文关键词	Cyanobacteria ISRU Mars life support perchlorate
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000607395700002
WOS关键词	BIOLOGICAL LIFE-SUPPORT ; RADIATION-RESISTANCE ; SYNTHETIC BIOLOGY ; CHROOCOCCIDIOPSIS ; DESICCATION ; EARTH ; TECHNOLOGIES ; STABILITY ; SURVIVORS ; SUCROSE
WOS类目	Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
WOS研究方向	Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/347954
作者单位	[Billi, Daniela; Fernandez, Beatriz Gallego; Fagliarone, Claudia] Univ Roma Tor Vergata, Dept Biol, Lab Astrobiol & Mol Biol Cyanobacteria, Rome, Italy; [Chiavarini, Salvatore] ENEA Casaccia, SSPT PROTER Div, Rome, Italy; [Rothschild, Lynn Justine] NASA, Ames Res Ctr, Space Sci & Astrobiol Div, Moffett Field, CA 94035 USA
推荐引用方式 GB/T 7714	Billi, Daniela,Fernandez, Beatriz Gallego,Fagliarone, Claudia,et al. Exploiting a perchlorate-tolerant desert cyanobacterium to support bacterial growth for in situ resource utilization on Mars[J],2021,20(1):29-35.
APA	Billi, Daniela,Fernandez, Beatriz Gallego,Fagliarone, Claudia,Chiavarini, Salvatore,&Rothschild, Lynn Justine.(2021).Exploiting a perchlorate-tolerant desert cyanobacterium to support bacterial growth for in situ resource utilization on Mars.INTERNATIONAL JOURNAL OF ASTROBIOLOGY,20(1),29-35.
MLA	Billi, Daniela,et al."Exploiting a perchlorate-tolerant desert cyanobacterium to support bacterial growth for in situ resource utilization on Mars".INTERNATIONAL JOURNAL OF ASTROBIOLOGY 20.1(2021):29-35.