干旱区生态环境知识资源中心(Arid): High Culturable Bacterial Diversity From a European Desert: The Tabernas Desert

Arid

DOI	10.3389/fmicb.2020.583120
	High Culturable Bacterial Diversity From a European Desert: The Tabernas Desert
	Molina-Menor, Esther; Gimeno-Valero, Helena; Pascual, Javier; Pereto, Juli; Porcar, Manuel
通讯作者	Porcar, M (corresponding author), Univ Valencia, CSIC, Inst Integrat Syst Biol I2SysBio, Paterna, Spain. ; Porcar, M (corresponding author), Univ Valencia, Darwin Bioprospecting Excellence SL, Parc Cient, Paterna, Spain.
来源期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
出版年	2021
卷号	11
英文摘要	One of the most diverse ecological niches for microbial bioprospecting is soil, including that of drylands. Drylands are one of the most abundant biomes on Earth, but extreme cases, such as deserts, are considered very rare in Europe. The so-called Tabernas Desert is one of the few examples of a desert area in continental Europe, and although some microbial studies have been performed on this region, a comprehensive strategy to maximize the isolation of environmental bacteria has not been conducted to date. We report here a culturomics approach to study the bacterial diversity of this dryland by using a simple strategy consisting of combining different media, using serial dilutions of the nutrients, and using extended incubation times. With this strategy, we were able to set a large (254 strains) collection of bacteria, the majority of which (93%) were identified through 16S ribosomal RNA (rRNA) gene amplification and sequencing. A significant fraction of the collection consisted of Actinobacteria and Proteobacteria, as well as Firmicutes strains. Among the 254 isolates, 37 different genera were represented, and a high number of possible new taxa were identified (31%), of which, three new Kineococcus species. Moreover, 5 out of the 13 genera represented by one isolate were also possible new species. Specifically, the sequences of 80 isolates held a percentage of identity below the 98.7% threshold considered for potentially new species. These strains belonged to 20 genera. Our results reveal a clear link between medium dilution and isolation of new species, highlight the unexploited bacterial biodiversity of the Tabernas Desert, and evidence the potential of simple strategies to yield surprisingly large numbers of diverse, previously unreported, bacterial strains and species.
英文关键词	microbial diversity Tabernas Desert drylands ecology biocrust Actinobacteria
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000609518800001
WOS关键词	SP NOV. ; GENOME SEQUENCE ; GEN. NOV. ; STRAIN ; ACTINOBACTERIA ; ALPHAPROTEOBACTERIUM ; BIODEGRADATION ; BIOREMEDIATION ; PSEUDONOCARDIA ; DEGRADATION
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/347907
作者单位	[Molina-Menor, Esther; Pereto, Juli; Porcar, Manuel] Univ Valencia, CSIC, Inst Integrat Syst Biol I2SysBio, Paterna, Spain; [Gimeno-Valero, Helena; Pascual, Javier; Pereto, Juli; Porcar, Manuel] Univ Valencia, Darwin Bioprospecting Excellence SL, Parc Cient, Paterna, Spain; [Pereto, Juli] Univ Valencia, Dept Bioquim & Biol Mol, Burjassot, Spain
推荐引用方式 GB/T 7714	Molina-Menor, Esther,Gimeno-Valero, Helena,Pascual, Javier,et al. High Culturable Bacterial Diversity From a European Desert: The Tabernas Desert[J],2021,11.
APA	Molina-Menor, Esther,Gimeno-Valero, Helena,Pascual, Javier,Pereto, Juli,&Porcar, Manuel.(2021).High Culturable Bacterial Diversity From a European Desert: The Tabernas Desert.FRONTIERS IN MICROBIOLOGY,11.
MLA	Molina-Menor, Esther,et al."High Culturable Bacterial Diversity From a European Desert: The Tabernas Desert".FRONTIERS IN MICROBIOLOGY 11(2021).