干旱区生态环境知识资源中心(Arid): Purification and characterization of two thermostable xylanases from a halotolerant Bacillus sp. Asc6BA isolated from Salar de Ascotan, Atacama Desert

Arid

DOI	10.1007/s00792-020-01210-z
	Purification and characterization of two thermostable xylanases from a halotolerant Bacillus sp. Asc6BA isolated from Salar de Ascotan, Atacama Desert
	Contreras, Francisca; Amenabar, Maximiliano J.; Blamey, Jenny M.
通讯作者	Blamey, JM (corresponding author), Fdn Cient & Cultural Biociencia, Jose Domingo Canas 2280, Santiago, Chile. ; Blamey, JM (corresponding author), Univ Santiago Chile, Fac Quim & Biol, Ave Libertador Bernardo OHiggins 3363, Santiago, Chile.
来源期刊	EXTREMOPHILES
ISSN	1431-0651
EISSN	1433-4909
出版年	2021
卷号	25 期号:1 页码:51-59
英文摘要	Two extracellular xylanases, denominated X2 and X3, were purified and characterized from the halotolerant bacterium Bacillus sp. Asc6BA isolated from Salar de Ascotan in the Atacama Desert. Xylanases were purified by anion exchange, cation exchange and size exclusion liquid chromatography. Xylanase X2 and X3 were purified similar to 690-fold and similar to 629-fold, respectively, compared to the concentrated extracellular fraction with a final specific activity of 169 and 154 u mg(-1), respectively. Optimal conditions of pH and temperature of xylanolytic activity were 6.0 and 60 degrees C for X2 and 7.0 and 60 degrees C for X3. Half-life of X2 xylanase was 30 min at 50 degrees C, while X3 xylanase was remarkably more thermostable, retaining more than 70% of its activity after 32 h of incubation at 50 degrees C. X2 exhibited K-m, V-max and k(cat) values of 7.17 mg mL(-1), 1.28 mM min(-1) mg(-1) and 425.33 s(-1), respectively. X3 exhibited K-m, V-max and k(cat) values of 6.00 mg mL(-1), 19.25 mM min(-1) mg(-1) and 82,515 s(-1), respectively. In addition to their thermal stabilities, these enzymes were shown to be resistant to freeze-drying. These stability properties, in addition to the ability of these enzymes to be active in a wide range of temperatures and pHs, make these xylanases good candidates for industrial applications.
英文关键词	Xylanase Salar de ascotan Birchwood xylan Atacama desert Pulp and paper Thermostability
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000604811200001
WOS关键词	MICROBIAL XYLANASES ; PRETREATMENT ; PULP
WOS类目	Biochemistry & Molecular Biology ; Microbiology
WOS研究方向	Biochemistry & Molecular Biology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/347880
作者单位	[Contreras, Francisca; Amenabar, Maximiliano J.; Blamey, Jenny M.] Fdn Cient & Cultural Biociencia, Jose Domingo Canas 2280, Santiago, Chile; [Blamey, Jenny M.] Univ Santiago Chile, Fac Quim & Biol, Ave Libertador Bernardo OHiggins 3363, Santiago, Chile
推荐引用方式 GB/T 7714	Contreras, Francisca,Amenabar, Maximiliano J.,Blamey, Jenny M.. Purification and characterization of two thermostable xylanases from a halotolerant Bacillus sp. Asc6BA isolated from Salar de Ascotan, Atacama Desert[J],2021,25(1):51-59.
APA	Contreras, Francisca,Amenabar, Maximiliano J.,&Blamey, Jenny M..(2021).Purification and characterization of two thermostable xylanases from a halotolerant Bacillus sp. Asc6BA isolated from Salar de Ascotan, Atacama Desert.EXTREMOPHILES,25(1),51-59.
MLA	Contreras, Francisca,et al."Purification and characterization of two thermostable xylanases from a halotolerant Bacillus sp. Asc6BA isolated from Salar de Ascotan, Atacama Desert".EXTREMOPHILES 25.1(2021):51-59.