干旱区生态环境知识资源中心(Arid): Non-Invasive Monitoring of Adrenocortical Activity in Three Sympatric Desert Gerbil Species

Arid

DOI	10.3390/ani11010075
	Non-Invasive Monitoring of Adrenocortical Activity in Three Sympatric Desert Gerbil Species
	Navarro-Castilla, Alvaro; Garrido, Mario; Hawlena, Hadas; Barja, Isabel
通讯作者	Navarro-Castilla, A (corresponding author), Autonomous Univ Madrid, Dept Biol, Ethophysiol Grp, Unit Zool,Fac Sci, E-28049 Madrid, Spain.
来源期刊	ANIMALS
ISSN	2076-2615
出版年	2021
卷号	11 期号:1
英文摘要	Simple Summary In this era, characterized by remarkable anthropogenic impacts on wildlife, it is crucial to monitor the health of wild animal populations while minimizing the interference to them. To this end, for a better understanding of the eco-physiology of wild animals, the adrenocortical activity can be non-invasively evaluated by measuring glucocorticoid metabolites excreted in feces. However, to ensure that the endocrine information is reliable, the experimental assays should be first validated and the causes for the major variability among individuals should be considered. Here we validated an enzyme immunoassay for measuring fecal corticosterone metabolites (FCM) in three wild gerbil species and emphasized the differences among them. These are endangered species, which play a key role in psammophilic communities, and provide a model system for various aspects in ecology. Thus, this work constitutes the first step toward using the FCMs of these species as indicators for individual and community stress. The study of the endocrine status can be useful to understand wildlife responses to the changing environment. Here, we validated an enzyme immunoassay (EIA) to non-invasively monitor adrenocortical activity by measuring fecal corticosterone metabolites (FCM) in three sympatric gerbil species (Gerbillus andersoni, G. gerbillus and G. pyramidum) from the Northwestern Negev Desert's sands (Israel). Animals included into treatment groups were injected with adrenocorticotropic hormone (ACTH) to stimulate adrenocortical activity, while control groups received a saline solution. Feces were collected at different intervals and FCM were quantified by an EIA. Basal FCM levels were similar in the three species. The ACTH effect was evidenced, but the time of FCM peak concentrations appearance differed between the species (6-24 h post-injection). Furthermore, FCM peak values were observed sooner in G. andersoni females than in males (6 h and 18 h post-injection, respectively). G. andersoni and G. gerbillus males in control groups also increased FCM levels (18 h and 48 h post-injection, respectively). Despite the small sample sizes, our results confirmed the EIA suitability for analyzing FCM in these species as a reliable indicator of the adrenocortical activity. This study also revealed that close species, and individuals within a species, can respond differently to the same stressor.
英文关键词	adrenocorticotropic hormone ACTH challenge endocrine activity enzyme immunoassay fecal corticosterone metabolites Gerbil non-invasive monitoring
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000609676300001
WOS类目	Agriculture, Dairy & Animal Science ; Veterinary Sciences
WOS研究方向	Agriculture ; Veterinary Sciences
来源机构	Ben-Gurion University of the Negev
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/347692
作者单位	[Navarro-Castilla, Alvaro; Barja, Isabel] Autonomous Univ Madrid, Dept Biol, Ethophysiol Grp, Unit Zool,Fac Sci, E-28049 Madrid, Spain; [Garrido, Mario] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Jacob Blaustein Ctr Sci Cooperat, IL-849900 Midreshet Ben Gurion, Israel; [Hawlena, Hadas] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Swiss Inst Dryland Environm & Energy Res, Mitrani Dept Desert Ecol, IL-849900 Midreshet Ben Gurion, Israel; [Barja, Isabel] Autonomous Univ Madrid, Ctr Invest Biodivers & Cambio Global CIBC UAM, C Darwin 2, E-28049 Madrid, Spain
推荐引用方式 GB/T 7714	Navarro-Castilla, Alvaro,Garrido, Mario,Hawlena, Hadas,et al. Non-Invasive Monitoring of Adrenocortical Activity in Three Sympatric Desert Gerbil Species[J]. Ben-Gurion University of the Negev,2021,11(1).
APA	Navarro-Castilla, Alvaro,Garrido, Mario,Hawlena, Hadas,&Barja, Isabel.(2021).Non-Invasive Monitoring of Adrenocortical Activity in Three Sympatric Desert Gerbil Species.ANIMALS,11(1).
MLA	Navarro-Castilla, Alvaro,et al."Non-Invasive Monitoring of Adrenocortical Activity in Three Sympatric Desert Gerbil Species".ANIMALS 11.1(2021).