干旱区生态环境知识资源中心(Arid): Co-culture of Saccharomyces cerevisiae (VS3) and Pichia stipitis (NCIM 3498) enhances bioethanol yield from concentrated Prosopis juliflora hydrolysate

Arid

DOI	10.1007/s13205-020-02595-6
	Co-culture of Saccharomyces cerevisiae (VS3) and Pichia stipitis (NCIM 3498) enhances bioethanol yield from concentrated Prosopis juliflora hydrolysate
	Naseeruddin, Shaik; Desai, Suseelendra; Venkateswar Rao, L.
通讯作者	Desai, S (corresponding author), ICAR Cent Res Inst Dry Land Agr, Hyderabad 500059, India.
来源期刊	3 BIOTECH
ISSN	2190-572X
EISSN	2190-5738
出版年	2021
卷号	11 期号:1
英文摘要	Biphasic acid hydrolysates and enzymatic hydrolysates from carbohydrate-rich Prosopis juliflora, an invasive perennial deciduous shrub of semi-arid regions, were used for bioethanol production. Saccharomyces cerevisiae and Pichia stipitis were used for fermentation of hexoses and pentoses. P. juliflora acid hydrolysate with an initial sugar concentration of 18.70 +/- 0.16 g/L was concentrated to 33.59 +/- 0.52 g/L by vacuum distillation. The concentrated hydrolysate was pretreated and fermented by mono- and co-culture methods either singly or in combination with enzyme hydrolysate and ethanol yields were compared. Monoculture with S. cerevisiae (VS3) and S. cerevisiae (NCIM3455) yielded maximum ethanol of 36.6 +/- 1.83 g/L and 37.1 +/- 1.86 g/L with a fermentation efficiency of 83.94 +/- 4.20% and 84.20 +/- 4.21%, respectively, after 36 h of fermentation. The ethanol yield obtained was 0.428 +/- 0.02 g/g substrate and 0.429 +/- 0.02 g/g substrate with a productivity of 1.017 +/- 0.051 g/L/hand 1.031 +/- 0.052 g/L/h, respectively. P. stipitis (NCIM3498) yielded maximum ethanol of 24 g/L with ethanol yield of 0.455 +/- 0.02 g/g substrate and a productivity of 1.004 +/- 0.050 g/L/h after 24 h of fermentation. With concentrated acid hydrolysate as substrate, S. cerevisiae (VS3) produced ethanol of 8.52 +/- 0.43 g/L, whereas S. cerevisiae (NCIM3455) produced 5.96 +/- 0.30 g/L of ethanol. P.stipitis (NCIM3498) produced 4.52 +/- 0.23 g/L of ethanol by utilizing 14.66 +/- 0.87 g/L of sugars. Co-culture with S. cerevisiae (VS3) addition after 18 h of addition of P. stipitis (NCIM3498) to the mixture of concentrated acid hydrolysate and enzyme hydrolysate produced 13.86 +/- 0.47 g/L of ethanol with fermentation efficiency, ethanol yield and productivity of 87.54 +/- 0.54%, 0.446 +/- 2.36 g/g substrate and 0.385 +/- 0.014 g/L/h, respectively. Hence, it is concluded that co-culture with S. cerevisiae and P. stipitis is feasible, further scaling up of fermentation of P. juliflora substrate for bioethanol production.
英文关键词	Prosopis juliflora Lignocellulose Co-culture Bioethanol Fermentation efficiency Ethanol yield
类型	Article
语种	英语
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000606456100002
WOS关键词	ETHANOL-PRODUCTION ; ENZYMATIC SACCHARIFICATION ; SUGARCANE BAGASSE ; ACID-HYDROLYSIS ; FERMENTATION ; THERMOTOLERANT ; PRETREATMENT ; SUBSTRATE ; DETOXIFICATION ; SELECTION
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/347607
作者单位	[Naseeruddin, Shaik; Venkateswar Rao, L.] Osmania Univ, Dept Microbiol, Hyderabad 500007, India; [Desai, Suseelendra] ICAR Cent Res Inst Dry Land Agr, Hyderabad 500059, India
推荐引用方式 GB/T 7714	Naseeruddin, Shaik,Desai, Suseelendra,Venkateswar Rao, L.. Co-culture of Saccharomyces cerevisiae (VS3) and Pichia stipitis (NCIM 3498) enhances bioethanol yield from concentrated Prosopis juliflora hydrolysate[J],2021,11(1).
APA	Naseeruddin, Shaik,Desai, Suseelendra,&Venkateswar Rao, L..(2021).Co-culture of Saccharomyces cerevisiae (VS3) and Pichia stipitis (NCIM 3498) enhances bioethanol yield from concentrated Prosopis juliflora hydrolysate.3 BIOTECH,11(1).
MLA	Naseeruddin, Shaik,et al."Co-culture of Saccharomyces cerevisiae (VS3) and Pichia stipitis (NCIM 3498) enhances bioethanol yield from concentrated Prosopis juliflora hydrolysate".3 BIOTECH 11.1(2021).