Knowledge Resource Center for Ecological Environment in Arid Area
| 项目编号 | 31502000 |
| 紫花苜蓿FAD基因调控α-亚麻酸生物合成机制研究 | |
| 刘文献 | |
| 主持机构 | 兰州大学 |
| 开始日期 | 2016 |
| 结束日期 | 2018 |
| 资助经费 | 200000(CNY) |
| 项目类别 | 青年科学基金项目 |
| 资助机构 | CN-NSFC（国家自然科学基金） |
| 语种 | 中文 |
| 国家 | 中国 |
| 英文简介 | α-linolenic acid is essential for human health, and its deficiency in the diet is a major explanation for many diseases. Alfalfa (Medicago sativa subsp. sativa) is the most widely cultivated leguminous forage in the world, but its low content of α-linolenic acid directly affects the α-linolenic acid proportions and nutritional value of milk. The regulatory mechanism of α-linolenic acid biosynthesis in alfalfa is not clear to date. Δ12 fatty acid desaturases (FADs) and Δ15FADs are the key enzymes involved in the biosynthetic pathway of α-linolenic acid. Through high-throughput transcriptome sequencing and RACE methods, the full-length of 2 Δ12FAD and 2 Δ15FAD cDNA sequences were cloned from alfalfa. Based on this, we aim to analyze the expression pattern, subcellular localization and biological function of Δ12FAD and Δ15FAD genes, clarify the key genes and the regulatory mechanism of the α-linolenic acid biosynthesis in alfalfa, and create materials with high content of α-linolenic acid. The results in this research can provide important theoretical foundations for the improvement of alfalfa quality and nutritional value of dairy products. |
| 中文关键词 | 牧草 ; 功能基因 ; 表达调控 ; 分子育种 |
| 来源机构 | 兰州大学 |
| 资源类型 | 项目 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/347006 |
| 推荐引用方式 GB/T 7714 | 刘文献.紫花苜蓿FAD基因调控α-亚麻酸生物合成机制研究.2016. |
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