Knowledge Resource Center for Ecological Environment in Arid Area
| 项目编号 | 31500012 |
| 荒漠地衣石果衣真菌（Endocarpon pusillum）抗旱基因的功能分析及利用研究 | |
| 王延延 | |
| 主持机构 | 中国科学院微生物研究所 |
| 开始日期 | 2016 |
| 结束日期 | 2018 |
| 资助经费 | 200000(CNY) |
| 项目类别 | 青年科学基金项目 |
| 资助机构 | CN-NSFC（国家自然科学基金） |
| 语种 | 中文 |
| 国家 | 中国 |
| 英文简介 | Endocarpon pusillum is a lichen-forming fungus with strong ability of drought resistant, it will be a huge and potential superiority gene resource library for genetic improvements the drought-resistant capacity of plant. Here we propose isolation and functional characterization of E. pusillum drought-resistant genes using molecular biology and biochemistry approaches. First, we will elucidate the role of the important genes in drought-resistant related signal pathway and the genes screening from yeast expression library by examining their expression pattern and phenotypic analysis of transgenic yeast. Furthermore, we will employ multiple sequences alignment and gene truncated mutant construction approaches to clarify the function of each truncated mutant. The target genes will transferred into plant and based on their special amino acid sequence with drought-resistant function to modify the homologous gene in plant. Finally, we will compare and research the feasibility of two transgenic genetic approaches. The project is under the guidance of “Desert Biological Carpet Engineering” and “deserts turned to arable land”, the accomplishment of this project would establish theoretical basis to illuminate the special genes in lichen-forming fungal signal pathway, and provide deeper insight into the potential of utilization of lichen genetic resources. |
| 中文关键词 | 地衣 ; 地衣型真菌 ; 抗旱 ; 基因功能分析 ; 转基因植物 |
| 来源机构 | 中国科学院微生物研究所 |
| 资源类型 | 项目 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/346892 |
| 推荐引用方式 GB/T 7714 | 王延延.荒漠地衣石果衣真菌（Endocarpon pusillum）抗旱基因的功能分析及利用研究.2016. |
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