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| 项目编号 | 31500063 |
| 沙漠核爆区微生物新型RNA伴侣蛋白Csp相关功能域研究 | |
| 周正富 | |
| 主持机构 | 中国农业科学院生物技术研究所 |
| 开始日期 | 2016 |
| 结束日期 | 2018 |
| 资助经费 | 180000(CNY) |
| 项目类别 | 青年科学基金项目 |
| 资助机构 | CN-NSFC（国家自然科学基金） |
| 语种 | 中文 |
| 国家 | 中国 |
| 英文简介 | Csp (Cold shock proteins), as RNA chaperone, plays an important regulatory role in diverse cellular processes which aids adaptation to stress. The aromatic residues which arranged on RNP functional domain determine the specific regulatory function of Csp on post-transcriptional level. Previous studies shows that a specific Csps clade which share a novel RNP domain from high-nuclear-radiation metagenomes. Heterologous expression of the novel Csp can significantly enhance the tolerant ability of Deinococcus radiodurans to gamma-radiation and oxidative stress. However, little is known about the mechanism to stress response modulated by these novel Csp. In this study, we analyze the activity site of novel functional domain on Csp which comes from nuclear-explosion-area-metagenomes by homology modeling method. Then, combined the technology of site-directed mutagenesis with Deinococcus heterologous expression system, identify the function of Csp novel functional domain. Finally, we compare the expression of stress resistance genes to understand the novel Csp mediated regulated mechanism in response to nuclear radiation stresses. The results of this study may provide us with valuable insights into the fundamental mechanisms for Csp modulated stress response systems, and help to deeply understand the adaptive revolution mode in radiation-resistant organisms. |
| 中文关键词 | RNA-伴侣蛋白Csp ; 新型功能域 ; 异源表达 ; 定点突变 ; 辐射抗性 |
| 来源机构 | 中国农业科学院生物技术研究所 |
| 资源类型 | 项目 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/346885 |
| 推荐引用方式 GB/T 7714 | 周正富.沙漠核爆区微生物新型RNA伴侣蛋白Csp相关功能域研究.2016. |
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