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| 项目编号 | 31500064 |
| 枯草芽孢杆菌稳定期高效表达启动子P16功能元件的解析及优化 | |
| 刘晓青 | |
| 主持机构 | 中国农业科学院生物技术研究所 |
| 开始日期 | 2016 |
| 结束日期 | 2018 |
| 资助经费 | 180000(CNY) |
| 项目类别 | 青年科学基金项目 |
| 资助机构 | CN-NSFC（国家自然科学基金） |
| 语种 | 中文 |
| 国家 | 中国 |
| 英文简介 | Bacillus subtilis, is the ideal host for the production of many industrial enzymes. A strong and controllable promoter is one of the key factors for efficiently expressing heterologous protein. Stationary phase specific promoters are the ideal promoters for the genes expression system , as they could express genes without inducer. Analysis and optimization of these promoters' core elements would explain the molecular mechanism of the genes expression specifically at the stationary phase and has important theoretical significance and application prospects. The analysis of the transcriptome of B.subtilis during the growth revealed that some genes were specifically expressed during stationary phase. Among the stationary phase specific promoters，we found the promoter P16 which the transcription activity is higher than the widely used constitutive promoter P43. Therefore, this study will analyze the cis-regulatory element of the promoter P16 by scanning mutagenesis, random mutagenesis, and the two fluorescent protein genes reporter system. The library of stationary phase specific promoters which cover a range of promoter activities with these elements will be constructed. These promoters can efficiently produce heterologous proteins without inducer. This work could play an important role in simplifying the production process and reducing cost in industrial production. Meanwhile, the analysis of these cis-elements provides theoretical basis for the research of the stationary phase physiological regulation in B.subtilis. |
| 中文关键词 | 启动子体系优化 ; 转录调控 ; 顺式作用元件 ; 启动子文库 ; 原核表达系统 |
| 来源机构 | 中国农业科学院生物技术研究所 |
| 资源类型 | 项目 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/346872 |
| 推荐引用方式 GB/T 7714 | 刘晓青.枯草芽孢杆菌稳定期高效表达启动子P16功能元件的解析及优化.2016. |
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