Knowledge Resource Center for Ecological Environment in Arid Area
| 项目编号 | 31400437 |
| 节杆菌中海藻糖合成代谢响应冻土退化的分子机制研究 | |
| 陈熙明 | |
| 主持机构 | 中国科学院寒区旱区环境与工程研究所 |
| 开始日期 | 2015 |
| 结束日期 | 2017 |
| 资助经费 | 250000(CNY) |
| 项目类别 | 青年科学基金项目 |
| 资助机构 | CN-NSFC（国家自然科学基金） |
| 语种 | 中文 |
| 国家 | 中国 |
| 英文简介 | Strains of Arthrobacter species are among the most frequently bacterium to isolated in permafrost. Arthrobacter sp. have important potential applications (pollutant degradation, biological productivity, etc.) and are essential for the biogeochemical cycles in soil. Trehalose is a protection molecular, which is essential for the cold shock，heat shock and lots of stress protections in Arthrobacter. Previous studies showed there are 3 pathways of trehalose synthesis in Arthrobacter species and the strains are using different pathways to adapt different environments. However, which pathways are prefer to use when Arthrobacter survival in permafrost are still unknown. The upstream of Shule River,which is transition Permafrost of Qinghai–Tibetan Plateau, is the lifeline of herdsmen in Hexi. With the degradation of permafrost in this area, the grassland is degraded dramatically. This project will analysis the Arthrobacter in the soil samples, which are isolated in different types of permafrost and different seasons in the upstream of Shule River. Using the real-time PCR and the clone library of 16S-ITS-23S rRNA to analysis the spatial and temporal variation of Arthrobacter community structures in different type of permafrost. Analysis the levels of trehalose, the trehalose synthesis pathways and the flexibility of N-Loop of OtsA can help us to understand the molecular mechanisms of trehalose metabolism, which is in response to permafrost degradation, in Arthrobacter. To study the adaption mechanisms of microorganisms during permafrost degradation, as well as to provide a scientific basis for ecological restoration by microorganisms. |
| 来源机构 | 中国科学院西北生态环境资源研究院 |
| 资源类型 | 项目 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/346812 |
| 推荐引用方式 GB/T 7714 | 陈熙明.节杆菌中海藻糖合成代谢响应冻土退化的分子机制研究.2015. |
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