Knowledge Resource Center for Ecological Environment in Arid Area
| 项目编号 | 31372360 |
| 红砂响应干旱的泌盐特征及其分子基础研究 | |
| 包爱科 | |
| 主持机构 | 兰州大学 |
| 开始日期 | 2014 |
| 结束日期 | 2017 |
| 资助经费 | 830000(CNY) |
| 项目类别 | 面上项目 |
| 资助机构 | CN-NSFC（国家自然科学基金） |
| 语种 | 中文 |
| 国家 | 中国 |
| 英文简介 | Reaumuria soongorica, a super xerophytic salt-secretion plant, is a main forage plant at desert steppe in northwest China, as well as an important species for windbreak and sand-fixation. It is a highlighted academic issue that salt glands participate in the adaptation of R. soongorica to stress environments. Our recent study showed that salt-secreting plays important roles in drought adaptation of R. soongorica. However, very little is known about the mechanisms underlying salt-secretion participating in plant's response to drought. Moreover, previous studies demonstrated that plasma membrane Na+/H+ antiporter (SOS1) plays an important role in mediating cell Na+ efflux, but those studies mainly focused on the non-salt-gland cells. In the present project, the salt-secretion dynamics and real-time Na+ secretion rate will be further analyzed in R. soongorica subjected to osmotic stress. The RsSOS1 gene encoding the plasma membrane Na+/H+ antiporter will be cloned from R. soongorica and its transcript abundance under osmotic stress will be quantitatively analyzed by real-time RT-PCR. The temporal and spatial expression pattern of RsSOS1 in the salt gland will be accurately determined through RNA in situ hybridization assays. The changes of salt-secretion traits and drought resistance will be assayed in RNAi plants using RNA interference. The aims of this project are to determine salt-secreting characteristics in super xerophyte R. soongorica responding to drought and analyze the roles of Na+ secretion mediated by SOS1 in drought adaptation of R. soongorica. The expected results will lay theoretical basis for elucidating the mechanisms of xerophytic salt-secretion plants adapting to stress environments. |
| 中文关键词 | 超旱生植物 ; 红砂 ; 抗旱性 ; 泌盐 ; 质膜Na+/H+逆向转运蛋白 |
| 来源机构 | 兰州大学 |
| 资源类型 | 项目 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/345633 |
| 推荐引用方式 GB/T 7714 | 包爱科.红砂响应干旱的泌盐特征及其分子基础研究.2014. |
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