Arid
项目编号31460030
荒漠先锋植物羽毛针禾根鞘微生物氮素营养功能研究
田永芝
主持机构新疆师范大学
开始日期2015
结束日期2018
资助经费510000(CNY)
项目类别地区科学基金项目
资助机构CN-NSFC(国家自然科学基金)
语种中文
国家中国
英文简介A sand born drought-resistant plant, Stipagrostis pennata(Trin.) de Winter, could colonize pioneerly in desert during revegetation. One strategy for their environment adaptation was the rhizosheath formatted by root-hair, mucilaginous exudates, microbial components, and soil particles. Previous reports has confirmed that rhizosheath of the S. pennata surrounded almost all of the root hairs. So the rhizosheath must be as a transit depot for nutrient exchange between plant and environment. Studies have also shown that nitrogen-fixing bacteria were included in the microorganisms isolated from rhizosheath. Nitrogen deficiency was the second limiting factor except water in desert. Plants that could colonize pioneerly in there but has no nitrogen-fixing ability should have its own strategy for nitrogen. For the purpose to understand its strategy for nitrogen utilization, the microorganisms and nitrogenase diversity of rhizosheath would be analyzed by high-throughput sequencing with unique primers. Then, the plant would be cultured in laboratory and nitrogen fertilizers with gradient concentrations would be added. The diversity responses of main microorganism population and nifH gene to the fertilizer addition would be estimated by Quantitative PCR with unique primers that selected from the previous analyzes above. The pathway of nitrogen transmission between rhizosheath and the plant would be traced by isotope-labelling method with 15N. The objective of the project was to reveal the environment adaptive mechanism of the S. pennata based on its nitrogen nutrient strategy. And this would be helpful for the desert ecology restoration. The innovation of the project was that the mechanism of the nitrogen strategy of the plant was explored by molecular detection and 15N tracing.
来源机构新疆师范大学
资源类型项目
条目标识符http://119.78.100.177/qdio/handle/2XILL650/344311
推荐引用方式
GB/T 7714
田永芝.荒漠先锋植物羽毛针禾根鞘微生物氮素营养功能研究.2015.
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