Knowledge Resource Center for Ecological Environment in Arid Area
项目编号 | 31360094 |
盐胁迫下活性氧介导植物根生长作用机理研究 | |
杨颖丽 | |
主持机构 | 西北师范大学 |
开始日期 | 2014 |
结束日期 | 2017 |
资助经费 | 550000(CNY) |
项目类别 | 地区科学基金项目 |
资助机构 | CN-NSFC(国家自然科学基金) |
语种 | 中文 |
国家 | 中国 |
英文简介 | The northwest of China is the major salint-alkaline desert soil regions, which is one of the most serious environmental stresses impeding plant growth and limiting crop production. Stress conditions are known to induce rapid and increased generation of reactive oxygen species (ROS) including hydrogen peroxide (H2O2), which may lead to oxidative damage on plant cells. On the other hand, ROS as a signal molecule is involved in growth and developmental process as well as various physiological and biochemical responses in plants. And also, the determination of plant adaptation to different stress conditions is important to ecological reconstruction in the salint-alkaline desert of northwest china. Plant root systems are quite susceptible to different stress conditions. As an important organ, plant root not only possesses many important physiological functions including absorption, fixation, storage, synthesis, and reproduction, but also influences the plant's adaptation to the environment. However, little information is known about regulation mechanism of ROS generation on the root growth of desert halophytes under stress environments. L. aureum (L.) Hill is a typical desert halophyte with the strong ability to resist salinity and alkalinity, drought, wind and dust, and it can efficiently fix moving sands. Drought-tolerant Xihan 2 is a wheat species widely grown in the north-west of China. In this study, these species are used to investigate the relationship of ROS generation and root growth, to comprehensively analyze the changes of enzyme activity, protein content and gene expression involved in ROS production, and thus to determine the regulation function of ROS on root growth under stress conditions, through a number of control experiments combined with protein electrophoresis, organization/cell chemistry orientation method and fluorescence microscopy. On the basis of these analyses, further study mainly aims to evaluate the effects of ROS generation on the structure and components of cell wall in plant roots, and to explore endogenous signal molecules associated with ROS actions, and thus to reveal the regulation approach and mechanism of ROS on plant root growth. These studies aim to provide scientific basis and theoretical guidance for further investigating the roles and the regulation mechanism of ROS signaling as well as for carrying out molecular genetic manipulation. |
中文关键词 | 活性氧 ; 根生长 ; 盐胁迫 ; 氧化胁迫 ; 耐盐机制 |
来源机构 | 西北师范大学 |
资源类型 | 项目 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/344289 |
推荐引用方式 GB/T 7714 | 杨颖丽.盐胁迫下活性氧介导植物根生长作用机理研究.2014. |
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