Knowledge Resource Center for Ecological Environment in Arid Area
| 项目编号 | 31360149 |
| 银沙槐内生菌抗干旱胁迫促种子萌发调控方式研究 | |
| 安登第 | |
| 主持机构 | 新疆师范大学 |
| 开始日期 | 2014 |
| 结束日期 | 2017 |
| 资助经费 | 470000(CNY) |
| 项目类别 | 地区科学基金项目 |
| 资助机构 | CN-NSFC（国家自然科学基金） |
| 语种 | 中文 |
| 国家 | 中国 |
| 英文简介 | Ammodendron argenteum (Pall.) Kuntze, one most important plant for desert ecosystem, has distinctively characteristic on drought-resistance. But it has becomes endangered in recent decades. One of the threatening factors is its critical value of drought tolerance with 0.12 Mpa on germination. Our previous study indicating that inoculation with cultures of endobacteria isolated from A. argenteum could enhance the germination rate by 11 times as high as control groups under drought stress of -1.2 Mpa by polyethylene glycol (PEG). Effects of the inoculation was more than pure water for stress releases. Most of the isolates could produce indoleacetic acid (IAA) and proline under laboratory culture but has no relationship with germination enhancing. The results also indicated that the effects of germination enhancing were delayed for a few days, presuming that there have some regulations on osmotic or metabolic of the seed. For the purpose to understand the pathways of symbiotic enhancing on germination and the regulation on seed metabolic, the cultures of the isolates would be inoculated to the surface and deep sterilized seeds. With gradient drought stress with PEG 6000, the cultures, cells resuspension with PBS, and the supernatants of the isolates that cultured with general laboratory medium would be used. Germination rates and seed composition with or without inoculation would be estimated. And then, the seeds with different germination period would be grind as medium and the strains would be cultures with it as semi- in vivo test. The metabolites will be analyzed and the cultures also inoculated for seed germination. The results will be helpful for our understanding the pathway of the endophyte on germination, the enhancing chemicals, and metabolically regulations. And the enhancing mechanism of the seed resistance for desert would be play a foundation for the application of microorganisms on desert ecosystems protection and restoration. |
| 中文关键词 | 抗干旱胁迫 ; 种子萌发 ; 内生菌 ; 共生 ; 银沙槐 |
| 来源机构 | 新疆师范大学 |
| 资源类型 | 项目 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/344225 |
| 推荐引用方式 GB/T 7714 | 安登第.银沙槐内生菌抗干旱胁迫促种子萌发调控方式研究.2014. |
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