Knowledge Resource Center for Ecological Environment in Arid Area
| 项目编号 | 1244630 |
| Assessment of Molecular Mechanisms involved in Nitrogen Cost Minimization of Oligotrophic Microorganisms | |
| Joseph Grzymski | |
| 主持机构 | Nevada System of Higher Education, Desert Research Institute |
| 开始日期 | 2013-08-01 |
| 结束日期 | 2014-07-31 |
| 资助经费 | 50716(USD) |
| 项目类别 | Standard Grant |
| 资助机构 | US-NSF（美国国家科学基金会） |
| 项目所属计划 | Systems and Synthetic Biology |
| 语种 | 英语 |
| 国家 | 美国 |
| 英文简介 | Intellectual Merit: Nitrogen cost-minimization (the reduction of nitrogen in cellular structures, especially protein) is a common and important strategy for marine microbes that thrive in oligotrophic waters. This is evidenced by the most successful organisms having low G+C genomes and thus using amino acids that have fewer N atoms in side chains and by the necessary trade-off this strategy entails - that proteins are, on average, larger in mass (require more non-limiting carbon). This project's core hypothesis is that cost-minimized organisms have specific genomic and molecular adaptations that promote nitrogen thrift and maintain a slow and steady approach to growth. Specifically, these organisms have sacrificed potential efficiencies in translation and regulation to maintain growth under any condition. They grow slowly regardless of substrate availability - there are fundamental limitations in metabolic and cell processes. It is posited that slow and steady growth hard-wired into the genetic code alleviates the need for more complex transcriptional regulation and complex, protein intensive redox sensing pathways. The experimental plan will test this core hypothesis by quantifying the primary transcriptome and local translation rates of a strain of Prochlorococcus grown under high and low nitrogen conditions. Broader Impacts: Transcription and translation are fundamental cellular processes that help set maximum growth rates of an organism. New techniques will be applied to capture a detailed picture of the transcriptional landscape of Prochlorococcus, and this has the potential to yield transformative information on the relationships between cost minimization and transcriptional complexity for one of the most abundant microbes on earth. The data generated during this project will be a rich resource to researchers in many fields; all of the data generated during this project will be released to public databases. This project has graduate and middle school educational components. The extensive laboratory and computer work offers a diverse educational potential using cutting-edge laboratory and bioinformatic techniques. Public outreach will involve a continued effort by the PI and his lab to engage, promote and improve science education in Nevada's poorest schools. This work will also contribute to the further development of the NSF-EPSCoR infrastructure and research activities in Nevada. |
| 来源学科分类 | Biological Sciences |
| URL | https://www.nsf.gov/awardsearch/showAward?AWD_ID=1244630 |
| 资源类型 | 项目 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/341533 |
| 推荐引用方式 GB/T 7714 | Joseph Grzymski.Assessment of Molecular Mechanisms involved in Nitrogen Cost Minimization of Oligotrophic Microorganisms.2013. |
| 条目包含的文件 | 条目无相关文件。 | |||||
| 个性服务 |
| 推荐该条目 |
| 保存到收藏夹 |
| 导出为Endnote文件 |
| 谷歌学术 |
| 谷歌学术中相似的文章 |
| [Joseph Grzymski]的文章 |
| 百度学术 |
| 百度学术中相似的文章 |
| [Joseph Grzymski]的文章 |
| 必应学术 |
| 必应学术中相似的文章 |
| [Joseph Grzymski]的文章 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
