干旱区生态环境知识资源中心(Arid): 西北旱区甘草深色有隔内生真菌物种多样性及空间分布

Arid

	西北旱区甘草深色有隔内生真菌物种多样性及空间分布
其他题名	Species diversity and spatial distribution of dark septate endophytic fungi in Glycyrrhiza uralensis in arid area of Northwest China
	贺超; 陈晓玉; 王文全; 侯俊玲
来源期刊	菌物学报
ISSN	1672-6472
出版年	2020
卷号	39 期号:8 页码:1487-1501
中文摘要	为探明西北旱区甘草Glycyrrhiza uralensis根系深色有隔内生真菌(dark septate endophytes,DSE)生态分布和定殖状况,于2018年7月分别从甘肃安西、民勤和宁夏沙坡头地区采集甘草不同灌丛范围0-10cm和20-30cm土壤和根系样品,系统研究不同样地甘草灌丛内外DSE生态分布及其与土壤因子的相关性。结果表明,不同样地甘草均能被DSE高度侵染,形成典型深色有隔菌丝和微菌核结构。不同样地DSE定殖率差异显著,安西DSE总定殖率(43.34%)显著低于民勤(90%)和沙坡头(88.34%)样地。相关性分析表明,DSE定殖率主要与土壤速效磷、有机碳和碱性磷酸酶显著相关。共分离鉴定11属13种DSE,即Acrocalymma vagum、Alternaria longissima、Alternaria chlamydospora、Alternaria chlamydosporigena、 Paraphoma chrysanthemicola、Darksidea alpha、Niesslia aemula、Tricharina ochroleuca、Acremonium nepalense、Fusarium solani、Preussia sp.、Leptosphaeria oraemaris和Ulocladium sp.。其中Acrocalymma vagum是甘草根系DSE优势菌种,为民勤和沙坡头共有种,而安西样地DSE物种多样性最高,共7种。主成分分析表明,安西DSE物种组成在0-10cm和20-30cm不同灌丛范围存在显著差异;单因素方差分析显示,安西和沙坡头DSE菌丝定殖率在距主干0-10cm显著高于20-30cm,说明荒漠环境灌丛覆盖对DSE真菌群落存在显著影响。方差分解结果表明,DSE定殖受样地空间分布、灌丛覆盖和土壤因子共同影响(74.91%),而DSE种类组成更多依赖于样地异质性(84.46%)和土壤养分(87.82%)的作用。研究不同荒漠环境DSE定殖和群落组成差异,有助于充分理解DSE在旱区植物生长和植被恢复中的功能和意义。
英文摘要	In order to investigate the ecological distribution and colonization of dark septate endophytes (DSE) in the roots of Glycyrrhiza uralensis in the arid area of northwest China, soil samples and root samples were collected from Anxi and Minqin in Gansu, and Shapotou in Ningxia, in July 2018. Soil and root samples were collected in the limits of 0-10cm and 20-30cm around the main stem. The investigation showed that G. uralensis could be highly infected by DSE in different areas, forming typical dark colored septate hyphae and microsclerotia. The total DSE colonization rate in Anxi (43.34%) was significantly lower than that in Minqin (90%) and Shapotou (88.34%). The correlation analysis indicated that DSE colonization rate in roots of G. uralensis was significantly related to soil available phosphorus, organic carbon and alkaline phosphatase. Eleven genera and 13 species of DSE were isolated and identified, including Acrocalymma vagum, Alternaria longissima, Alternaria chlamydospora, Alternaria chlamydosporigena, Paraphoma chrysanthemicola, Darksidea alpha, Niesslia aemula, Tricharina ochroleuca, Acremonium nepalense, Fusarium solani, Preussia sp., Leptosphaeria oraemaris and Ulocladium sp. Acrocalymma vagum is dominant, being common in Minqin and Shapotou, while Anxi possesses the highest diversity of DSE, with seven species in total. Principal component analysis showed that in Anxi the species composition of DSE within the limits of 0-10cm was significantly different from that of 20-30cm. Single factor analysis of variance showed that the colonization rate of DSE hyphae in Anxi and Shapotou was significantly higher within the limits of 0-10cm from the main stem as compared with that within 20-30cm. There was a significant impact of plant cover degree on DSE community in desert ecological environment. Variation partitioning revealed that the colonization of DSE fungi was affected by the spatial distribution of sample plots, plant cover degree and soil factors (74.91%), while the species composition was more dependent on the heterogeneity of sample plots (84.46%) and soil nutrients (87.82%). Our results seem helpful to fully understand the function and significance of DSE in arid plant growth and vegetation restoration.
中文关键词	深色有隔内生真菌 ; 生态分布 ; 群落组成 ; 甘草 ; 干旱环境
英文关键词	dark septate endophytes ecological distribution community composition Glycyrrhiza uralensis arid environments
类型	Article
语种	中文
收录类别	CSCD
WOS研究方向	Agriculture
CSCD记录号	CSCD:6790471
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/336834
作者单位	贺超, 中国医学科学院北京协和医学院药用植物研究所, 北京 100193, 中国.; 陈晓玉, 中国医学科学院北京协和医学院药用植物研究所, 北京 100193, 中国.; 王文全, 中国医学科学院北京协和医学院药用植物研究所;;北京中医药大学中药学院, ;;, ;;, 北京;; 100193;;100029, 中国.; 侯俊玲, 北京中医药大学中药学院, 100029.
推荐引用方式 GB/T 7714	贺超,陈晓玉,王文全,等. 西北旱区甘草深色有隔内生真菌物种多样性及空间分布[J],2020,39(8):1487-1501.
APA	贺超,陈晓玉,王文全,&侯俊玲.(2020).西北旱区甘草深色有隔内生真菌物种多样性及空间分布.菌物学报,39(8),1487-1501.
MLA	贺超,et al."西北旱区甘草深色有隔内生真菌物种多样性及空间分布".菌物学报 39.8(2020):1487-1501.