干旱区生态环境知识资源中心(Arid): 青海极端生境7株萎缩芽孢杆菌的生物活性

Arid

	青海极端生境7株萎缩芽孢杆菌的生物活性
其他题名	Biological activity of 7 Bacillus atrophaeus strains isolated under extreme environment in Qinghai
	杨雪; 谢永丽; 陈兰; 吴晓晖; 武玲玲; 孟晓霞; 夏介卓玛
来源期刊	福建农林大学学报. 自然科学版
ISSN	1671-5470
出版年	2020
卷号	49 期号:4 页码:459-466
中文摘要	通过生理生化特征测定、16S rDNA和gyrB基因序列分析对分离自青海海西州托素湖盐碱地黑枸杞根围、大格勒荒漠沙地红柳根围、茶卡盐湖周边骆驼蓬根围的7株芽孢杆菌进行鉴定;采用平板对峙法测定菌株拮抗病原真菌活性; MALDI-TOFMS质谱测定分析菌株的抑菌活性物质;用CMC法检测菌株降解纤维素活性,并检测菌株的耐盐性和耐低温性;用改良阿须贝(Ashby)无氮培养基检测菌株固氮活性.结果表明: 7株菌株TSH5、DGLWZ10、DCD1、CKL1、CKL4、CKL5、CKL6均被鉴定为萎缩芽孢杆菌(Bacillus atrophaeus); 7株菌株对病原真菌油菜菌核病菌(Sclerotinia sclerotiorum)和瓜类枯萎病菌(Fusarium oxysporum)均具有拮抗活性(抑菌圈平均直径均>16 mm);菌株DCD1可产生脂肽类化合物surfactin、fengycin、bacillomycins D,菌株CKL1可产生脂肽类化合物surfactin、fengycin;菌株DCD1、CKL1、CKL4、CKL5、CKL6具有不同程度的降解纤维素活性;菌株CKL1、CKL4、CKL5、CKL6能在含13% NaCl的平板上正常生长,在低温4 ℃的平板上缓慢生长,表现出较强的耐盐性及低温适生性;菌株DGLWZ10在改良Ashby无氮液体培养基中可正常生长,具有固氮活性.
英文摘要	Biochemical characteristics, 16S rDNA and gyrB gene sequences analysis were used to identify 7 Bacillus strains isolated from the rhizosphere of Lycium ruthenicum grown in the saline-alkali soil around Tuosu Lake,Tamarix ramosissima from the desert of Haixi Prefecture,and Peganum grown around Chaka Salt Lake,Qinghai Province. The antagonistic activities were determined by plate confrontation experiment,and the antibacterial substances were detected using MALDI-TOF-MS mass spectrometry. The cellulose degradation activities of the strains were tested by CMC method. Salt tolerance was evaluated using NaCl solution and low temperature resistance were tested under 18 ℃. Nitrogen fixation activities were determined using Ashby's nitrogen-free liquid medium. The results showed that 7 strains of TSH5,DGLWZ10,DCD1,CKL1,CKL4,CKL5,CKL6 were identified as Bacillus atrophaeus and had antagonistic activities against pathogenic fungi Sclerotinia sclerotiorum and Fusarium oxysporum,with inhibition zone over 16 mm. The strain DCD1 can produce lipopeptide compounds of surfactin,fengycin and bacillomycins D,and the strain CKL1 can produce surfactin and fengycin. Strains of DCD1,CKL1,CKL4,CKL5 and CKL6 can degrade cellulose to various degrees. Strains of CKL1,CKL4,CKL5 and CKL6 were able to grow normally on plates with 13% NaCl and grow slowly at 4 ℃,indicating satisfactory salt tolerance and low temperature adaptability. The strain DGLWZ10 grew normally in the modified Ashby nitrogen-free liquid medium,indicating its nitrogen-fixing activity.
中文关键词	青海 ; 极端生境 ; 萎缩芽孢杆菌 ; 拮抗活性 ; 纤维素降解活性 ; 脂肽类化合物
英文关键词	Qinghai Province extreme environment Bacillus atrophaeus antagonistic activity cellulose degradation lipopeptides
类型	Article
语种	中文
收录类别	CSCD
WOS研究方向	Agriculture
CSCD记录号	CSCD:6790275
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/336833
作者单位	杨雪, 青海大学;;青海省高寒草地适应性管理重点实验室, 青海省省部共建三江源生态与高原农牧业国家重点实验室;;青海省高寒草地适应性管理重点实验室, ;;西宁, ;;青海 ;;810016.; 谢永丽, 青海大学;;青海省高寒草地适应性管理重点实验室, 青海省省部共建三江源生态与高原农牧业国家重点实验室;;青海省高寒草地适应性管理重点实验室, ;;西宁, ;;青海 ;;810016.; 陈兰, 青海大学, 青海省省部共建三江源生态与高原农牧业国家重点实验室.; 吴晓晖, 青海大学, 青海省省部共建三江源生态与高原农牧业国家重点实验室.; 武玲玲, 青海大学, 青海省省部共建三江源生态与高原农牧业国家重点实验室.; 孟晓霞, 青海大学, 青海省省部共建三江源生态与高原农牧业国家重点实验室.; 夏介卓玛, 青海大学, 青海省省部共建三江源生态与高原农牧业国家重点实验室.
推荐引用方式 GB/T 7714	杨雪,谢永丽,陈兰,等. 青海极端生境7株萎缩芽孢杆菌的生物活性[J],2020,49(4):459-466.
APA	杨雪.,谢永丽.,陈兰.,吴晓晖.,武玲玲.,...&夏介卓玛.(2020).青海极端生境7株萎缩芽孢杆菌的生物活性.福建农林大学学报. 自然科学版,49(4),459-466.
MLA	杨雪,et al."青海极端生境7株萎缩芽孢杆菌的生物活性".福建农林大学学报. 自然科学版 49.4(2020):459-466.