干旱区生态环境知识资源中心(Arid): 干旱区沙生药用植物锁阳土壤微生物群落分析与功能预测

Arid

	干旱区沙生药用植物锁阳土壤微生物群落分析与功能预测
其他题名	Analysis and function prediction of soil microbial communities of Cynomorium songaricum in two daodi-origins
	孙晓; 林余霖; 李葆莉; 黄林芳
来源期刊	药学学报
ISSN	0513-4870
出版年	2020
卷号	55 期号:6 页码:1334-1344
中文摘要	我国不同道地产地的同种药材品质差异显著,课题组前期研究以原儿茶酸为化学标志物将国产锁阳分为长城外型(内蒙古)与长城内型(甘肃等)两个生态型,然而引起锁阳品质变异的生态机制尚不清楚。本文基于微生物组-生态策略,对两个生态型主要道地产区内蒙古和甘肃锁阳的根际土壤进行16s扩增子测序,分析不同产地锁阳土壤微生物群落的组成,并运用Tax4Fun对两个产地锁阳土壤微生物群落进行代谢功能预测,最后结合锁阳关键微生物组丰度及生态气候因子进行冗余分析和相关性分析。微生物组测序结果发现内蒙古锁阳土壤微生物群落多样性显著高于甘肃产区,发掘到5个共有核心微生物组(节杆菌属Arthrobacter、链霉菌属Streptomyces和芽孢杆菌属Bacillus等)及可区别两个产地土壤微生物群落的6个特异性biomarkers(微球茎属Microbulbifer、Methyloceanibacter和Cynomorium_coccineum等)。代谢功能预测首次展现了两个产地锁阳土壤微生物组的代谢功能谱。冗余分析和相关性分析结果均显示年日照时数是影响锁阳土壤微生物群落组成的主要生态因子,其与链霉菌属和芽孢杆菌属呈极显著负相关。本文为阐释锁阳两大道地产区品质变异的形成机制提供了新思路,也为中药品质生态学理论研究提供科学依据。
英文摘要	The quality of the same herbal medicines in different daodi-origins in China is significantly different.Our research team previously found that using protocatechuic acid as a chemical marker can divide the Cynomorium songaricum into outside-the GreatWall(InnerMongolia)and inside-the GreatWall(Gansu).However,the ecological mechanism that causes the quality variation of C.songaricum is unclear.Based on the microbiome-ecological strategy,16s amplicon sequencing was performed on rhizosphere soils of C.songaricum in two origins(Inner Mongolia and Gansu),and the composition of soil microbial communities was analyzed.Tax4Fun was used to predict the metabolic function of soil microbial communities.Correlation analysis by combing with the abundance of key microbial community and ecological climate factors was performed.Microbiome sequencing results revealed that the diversity of soil microbial communities in Inner Mongolia was significantly higher than that in Gansu.Five core microbiomes(Arthrobacter,Streptomyces,Bacillus,etc.)and six specific biomarkers of soil microbial communities(Microbulbifer,Methyloceanibacter,Cynomorium_coccineum,etc.)which could be distinguished from two origins were identified.For the first time,metabolic function prediction showed the metabolic function profile of soil microbiome of C.songaricum in two origins.Redundancy analysis and correlation analysis results showed that the annual sunshine hours was the main ecological factor affecting the composition of the soil microbial community of C.songaricum,and it was extremely significantly negatively related to Streptomyces and Bacillus.This article provides a new idea for explaining the formation mechanism of quality variation in the daodi-origins of C.songaricum,and also provides a scientific basis for theoretical research on the quality ecology of herbal medicines.
中文关键词	锁阳 ; 中药品质生态学 ; 土壤微生物群落 ; 16s扩增子测序 ; 道地药材
英文关键词	Cynomorium songaricum quality ecology on Chinese medicines soil microbial community 16s amplicon sequencing Daodi herb
类型	Article
语种	中文
收录类别	CSCD
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:6770440
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/336720
作者单位	孙晓, 中国医学科学院、北京协和医学院药用植物研究所;;中药资源教育部工程研究中心, 国家中医药管理局中药资源保护重点研究室;;中药资源教育部工程研究中心, ;;, 北京;;北京 100193;;100193, 中国.; 黄林芳, 中国医学科学院、北京协和医学院药用植物研究所;;中药资源教育部工程研究中心, 国家中医药管理局中药资源保护重点研究室;;中药资源教育部工程研究中心, ;;, 北京;;北京 100193;;100193, 中国.; 林余霖, 中国医学科学院、北京协和医学院药用植物研究所, 国家中医药管理局中药资源保护重点研究室, 北京 100193, 中国.; 李葆莉, 中国医学科学院、北京协和医学院药用植物研究所, 国家中医药管理局中药资源保护重点研究室, 北京 100193, 中国.
推荐引用方式 GB/T 7714	孙晓,林余霖,李葆莉,等. 干旱区沙生药用植物锁阳土壤微生物群落分析与功能预测[J],2020,55(6):1334-1344.
APA	孙晓,林余霖,李葆莉,&黄林芳.(2020).干旱区沙生药用植物锁阳土壤微生物群落分析与功能预测.药学学报,55(6),1334-1344.
MLA	孙晓,et al."干旱区沙生药用植物锁阳土壤微生物群落分析与功能预测".药学学报 55.6(2020):1334-1344.