干旱区生态环境知识资源中心(Arid): 生防细菌ML-3抗菌蛋白发酵条件的优化及其防治应用

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	生防细菌ML-3抗菌蛋白发酵条件的优化及其防治应用
其他题名	Optimization of Fermentation Conditions of Biocontrol Bacterium ML-3 Antifungal Protein and Its Application
	李静舒; 赵佳
来源期刊	生物技术通报
ISSN	1002-5464
出版年	2020
卷号	36 期号:6 页码:83-92
中文摘要	明确菌株ML-3抗菌蛋白对马铃薯早疫病的防效效果,为马铃薯早疫病生物防治提供参考依据。本研究采用硫酸铵分级沉淀、琼脂扩散法、正交试验、田间试验以及16S rDNA系统进化分析相结合的方法对菌株ML-3产抗菌蛋白的较优碳源、氮源、发酵条件、最佳硫酸铵沉淀浓度、抑菌稳定性、马铃薯早疫病田间防效和分类地位进行了研究。结果表明:该菌株产生的抗菌蛋白最佳发酵液配方为2%马铃薯、1%蛋白胨和0.5%NaCl,最佳发酵条件为:初始pH值7.0、温度30℃、装液量400 mL/500 mL、接种量5 mL、发酵48 h;抗菌蛋白在温度≤ 60℃、pH值5-8和紫外照射36 h内具有良好稳定性,而且对K~+、Fe~(2+)、Zn~(2+)、Na~+稳定性较好;马铃薯早疫病防治的田间用药浓度为≥ 10 mg/L;使用16S rDNA序列可将该菌株鉴定为枯草芽孢杆菌沙漠亚种(Bacillus subtilis subsp. inaquosorum)。说明菌株ML-3抗菌蛋白在防治马铃薯早疫病和生物农药开发中应用潜力明显。
英文摘要	In order to clarify the control effect of antifungal protein of strain ML-3 on potato early blight and to provide reference for biological control of potato early blight,the methods of ammonium sulfate fractionation,agar diffusion,orthogonal test,field test and 16S rDNA sequence analysis were used to study the optimal carbon source,nitrogen source,fermentation conditions,optimal ammonium sulfate precipitation concentration,bacteriostatic stability,field control effect and taxonomic status of strain ML-3. The results showed that the optimal composition of fermentation broth was 2% potato,1% peptone and 0.5% NaCl. The optimal fermentation conditions were initial pH 7.0,temperature 30℃,and fermentation liquid 400 mL in 500 mL Erlenmeyer flasks,inoculation volume 5 mL,and fermentation 48 h. In addition,the antifungal protein presented fine stability,when temperature ≤ 60℃,pH value from 5 to 8,and UV irradiation 36 h. The antifungal protein also had favorable stability to K~+,Fe~(2+),Zn~(2+),and Na~+. Besides,the concentration of antifungal protein should be ≥ 10 mg/L when it was used to control potato early blight in field. Strain ML-3 was identified as Bacillus subtilis subsp. inaquosorum based on 16S rRNA sequence analysis. All results indicate that the antifungal protein of strain ML-3 demonstrates obvious application potential in controlling potato early blight and the development bio-pesticide.
中文关键词	马铃薯早疫病 ; 抗菌蛋白 ; 发酵优化 ; 生防细菌ML-3 ; 田间防效
英文关键词	potato early blight antifungal protein optimization of fermentation bio-control bacterium ML-3 field control effect
类型	Article
语种	中文
收录类别	CSCD
WOS研究方向	Agriculture
CSCD记录号	CSCD:6766508
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/336708
作者单位	李静舒, 山西广播电视大学, 太原, 山西 030027, 中国.; 赵佳, 山西省农业科学院生物技术研究中心, 太原, 山西 030031, 中国.
推荐引用方式 GB/T 7714	李静舒,赵佳. 生防细菌ML-3抗菌蛋白发酵条件的优化及其防治应用[J],2020,36(6):83-92.
APA	李静舒,&赵佳.(2020).生防细菌ML-3抗菌蛋白发酵条件的优化及其防治应用.生物技术通报,36(6),83-92.
MLA	李静舒,et al."生防细菌ML-3抗菌蛋白发酵条件的优化及其防治应用".生物技术通报 36.6(2020):83-92.