干旱区生态环境知识资源中心(Arid): 基于高通量测序的准噶尔盆地荒漠土壤细菌多样性及群落结构特征

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	基于高通量测序的准噶尔盆地荒漠土壤细菌多样性及群落结构特征
其他题名	A high-throughput sequencing evaluation of bacterial diversity and community structure of the desert soil in the Junggar Basin
	魏鹏; 安沙舟; 董乙强; 孙宗玖; 别尔达吾列提·希哈依; 李超
来源期刊	草业学报
ISSN	1004-5759
出版年	2020
卷号	29 期号:5 页码:182-190
中文摘要	荒漠草地占新疆草地总面积的46.9%,不同荒漠类型的气候差异显著,植被和土壤类型也各不相同。以准噶尔盆地大面积分布的3种代表性荒漠植被类型(南缘伊犁绢蒿荒漠、腹地白梭梭荒漠和北缘盐生假木贼荒漠)为研究对象,应用高通量测序技术,分析比较3种荒漠植被类型的土壤细菌群落组成和多样性特征。结果表明:放线菌门、变形菌门、绿弯菌门、酸杆菌门、芽单胞菌门和拟杆菌门是准噶尔盆地荒漠土壤中6个主要优势类群细菌,其相对丰度累计超过94%。放线菌门在3种荒漠类型中相对丰度最高,均超过50%,但差异不显著(P>0.05);酸杆菌门在伊犁绢蒿荒漠中相对丰度最高,厚壁菌门在白梭梭荒漠丰度最高,拟杆菌门在盐生假木贼荒漠丰度最高,且这3种菌门在3种荒漠类型中差异显著(P<0.05);在3种荒漠类型中,白梭梭荒漠细菌操作分类单元(OTU)数最少,多样性最低。Pearson相关性分析和RDA分析结果均表明,年均降水量和土壤有机碳是影响细菌群落结构组成和多样性最显著的环境因子。
英文摘要	Desert grassland accounts for 46.9% of the total grassland area in Xinjiang,with different desert types in areas of different climate,each with significantly different vegetation and soil types.Three representative desert vegetation types:Seriphidium transiliense desert in the southern margin,Haloxylon persicum desert in the hinterland and Anabasis salsa desert in the northern margin of the Junggar Basin,were chosen for study.The bacterial community composition and diversity characteristics of the three desert vegetation types were analyzed and compared using a high-throughput sequencing technique.It was found that Actinobacteria, Proteobacteria,Chloroflexi,Acidobacteria,Gemmatimonadetes,and Bacteroidetes were the six dominant groups of bacteria in these Junggar Basin soils.The cumulative abundance was more than 94%.Actinobacteria had the highest relative abundance(>50%)in all three desert types,and the difference between desert types for relative abundance of Actinobacteria was not significant(P>0.05).Acidobacteria had a relative abundance in S.transiliense desert notably higher than in other desert types,while Firmicutes bundance was higher in H. persicum desert than in other desert types,and Bacteroidetes had the highest relative abundance in A.salsa desert,and these differences were significant(P<0.05).Among the three desert types,the number of bacterial operational taxonomic units in H. persicum desert was the lowest and the community diversity was the lowest. Pearson correlation and redundancy analyses showed that average annual precipitation and soil organic carbon are the most significant environmental factors affecting the composition and diversity of the bacterial communities studied.
中文关键词	准噶尔荒漠 ; 高通量测序 ; 土壤细菌 ; 土壤养分
英文关键词	Junggar desert high-throughput sequencing soil bacteria soil nutrients
类型	Article
语种	中文
收录类别	CSCD
WOS研究方向	Agriculture
CSCD记录号	CSCD:6718704
来源机构	新疆农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/336459
作者单位	魏鹏, 新疆农业大学草业与环境科学学院;;新疆畜牧科学院草业研究所, ;;, 乌鲁木齐;;乌鲁木齐, 新疆;;新疆 830052;;830011, 中国.; 安沙舟, 新疆农业大学草业与环境科学学院, 乌鲁木齐, 新疆 830052, 中国.; 董乙强, 新疆农业大学草业与环境科学学院, 乌鲁木齐, 新疆 830052, 中国.; 孙宗玖, 新疆农业大学草业与环境科学学院, 乌鲁木齐, 新疆 830052, 中国.; 别尔达吾列提·希哈依, 新疆农业大学草业与环境科学学院, 乌鲁木齐, 新疆 830052, 中国.; 李超, 新疆畜牧科学院草业研究所, 乌鲁木齐, 新疆 830011, 中国.
推荐引用方式 GB/T 7714	魏鹏,安沙舟,董乙强,等. 基于高通量测序的准噶尔盆地荒漠土壤细菌多样性及群落结构特征[J]. 新疆农业大学,2020,29(5):182-190.
APA	魏鹏,安沙舟,董乙强,孙宗玖,别尔达吾列提·希哈依,&李超.(2020).基于高通量测序的准噶尔盆地荒漠土壤细菌多样性及群落结构特征.草业学报,29(5),182-190.
MLA	魏鹏,et al."基于高通量测序的准噶尔盆地荒漠土壤细菌多样性及群落结构特征".草业学报 29.5(2020):182-190.