干旱区生态环境知识资源中心(Arid): 基于高通量测序技术分析2种菌草根际土壤真菌群落多样性

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	基于高通量测序技术分析2种菌草根际土壤真菌群落多样性
其他题名	High-throughput Sequencing Analysis of Fungal Community in Rhizosphere Soils of Two Plants for Mushroon Cultivation (JUNCAO)
	叶文雨; 谢序泽; 许钰滢; 倪明月; 胡红莉; 余文英; Norvienyeku Justice; 鲁国东
来源期刊	热带作物学报
ISSN	1000-2561
出版年	2020
卷号	41 期号:3 页码:556-563
中文摘要	为了解福州菌草基地巨菌草和绿洲一号2种菌草的根际土壤真菌群落多样性,采用Illumina Miseq高通量测序技术和生物信息学方法,对菌草根际土真菌的多样性及群落结构进行分析。结果表明:从3份土壤样本中获得525 292条ITS序列,在97%序列相似性基础上可划分为7267个可操作分类单元(OTU)。真菌群落的丰富度指数(ACE)以菌草非根际土最低,绿洲一号根际土略高于巨菌草根际土;而多样性指数(Shannon-Wiener和Simpson)菌草根际土高于非根际土,绿洲一号根际土略高于巨菌草根际土。3份样品的优势菌门为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、接合菌门(Zygomycota)、壶菌门(Chytridiomycota)、球囊菌门(Glomeromycota);优势菌纲为散囊菌纲(Eurotiomycetes)、粪壳菌纲(Sordariomycetes)、座囊菌纲(Dothideomycetes)、伞菌纲(Agaricomycetes)、锤舌菌纲(Leotiomycetes)、圆盘菌纲(Orbiliomycetes)、银耳纲(Tremellomycetes)、粘膜菌纲(Wallemiomycetes);优势菌属为篮状菌属(Talaromyces)、深黄伞形霉属(Umbelopsis)、枝孢菌属(Cladosporium)、暗双孢菌属(Cordana)、镰刀菌属(Fusarium)、隐球菌属(Cryptococcus)、淡紫紫霉属(Purpureocillium)、赤霉菌属(Gibberella)、灵芝属(Ganoderma)。研究结果表明,菌草根际土真菌群落多样性高于非根际土,为进一步更好地利用菌草提供理论参考依据。
英文摘要	High-throughput sequencing and bioinformatics analysis were used to analyze the composition of fungal communities in the rhizosphere and non-rhizosphere soils from the plants for mushroom cultivation. A total of 525 292 fungal ITS sequences from the samples were obtained. 7267 OTU (operational taxonomic units) could be grouped based on 97% sequence similarity as the threshold. The ACE of the fungal community was the lowest in the non-rhizosphere soil, and the rhizosphere soil of Lvzhou No. 1 was slightly higher than that in the rhizosphere soil of the plants for mushroom cultivation. The Shannon-wiener and Simpson values obtained for rhizosphere soils were higher than the values recorded from non-rhizosphere soils. Also, the Shannon-wiener and Simpson values for Lvzhou No. 1 rhizosphere soil was slightly higher compared to the values obtained for the rhizosphere soil of the plants for mushroom cultivation. This study further revealed Ascomycota, Basidiomycota, Zygomycota, Chytridiomycota, and Glomeromycota as the pre-dominant phylum present in all the rhizosphere soil samples. From class level analysis, we identified eight pre-dominant classes in all soil samples, including Eurotiomycetes, Sordariomycetes, Agaricomycetes, Leotiomycetes, Orbiliomycetes, Tremellomycetes, Dothideomycetes, and Wallemiomycetes. Nine pre-dominant genuses, including Talaromyces, Umbelopsis, Cladosporium, Cordana, Fusarium, Cryptococcus, Purpureocillium, Gibberella, and Ganoderma in all the soil samples were revealed. The results would provide insights into the prevailing upsurge in diversity in the rhizosphere fungi community compared to the non-rhizosphere soil and would serve as a reference for the characterization of rhizosphere fungi.
中文关键词	菌草 ; 根际土壤 ; 真菌群落多样性 ; 高通量测序
英文关键词	plants for mushroom cultivation (JUNCAO) rhizosphere soil diversity of fungal communities highthroughput sequencing
类型	Article
语种	中文
收录类别	CSCD
WOS研究方向	Microbiology
CSCD记录号	CSCD:6700445
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/336377
作者单位	叶文雨, 国家菌草工程技术研究中心;;福建农林大学生命学院, 国家菌草工程技术研究中心;;, 福州;;福州, 福建;;福建 350002;;350002, 中国.; 谢序泽, 福建农林大学生命学院, 福州, 福建 350002, 中国.; 许钰滢, 福建农林大学生命学院, 福州, 福建 350002, 中国.; 余文英, 福建农林大学生命学院, 福州, 福建 350002, 中国.; 倪明月, 福建农林大学植物保护学院, 福州, 福建 350002, 中国.; 胡红莉, 福建农林大学植物保护学院, 福州, 福建 350002, 中国.; 鲁国东, 国家菌草工程技术研究中心;;福建农林大学植物保护学院, 国家菌草工程技术研究中心;;, 福州;;福州, 福建;;福建 350002;;350002, 中国.
推荐引用方式 GB/T 7714	叶文雨,谢序泽,许钰滢,等. 基于高通量测序技术分析2种菌草根际土壤真菌群落多样性[J],2020,41(3):556-563.
APA	叶文雨.,谢序泽.,许钰滢.,倪明月.,胡红莉.,...&鲁国东.(2020).基于高通量测序技术分析2种菌草根际土壤真菌群落多样性.热带作物学报,41(3),556-563.
MLA	叶文雨,et al."基于高通量测序技术分析2种菌草根际土壤真菌群落多样性".热带作物学报 41.3(2020):556-563.