Arid
Pyrosequencing of nirS gene revealed spatial variation of denitrifying bacterial assemblages in response to wetland desertification at Tibet plateau
Gu Yunfu; Liu Ting; Bai Yan; Xiang Quanju; Zhang Xiaoping; Chen Qiang
来源期刊Journal of Mountain Science
ISSN1672-6316
出版年2019
卷号16期号:5页码:1121-1134
英文摘要Amplicon sequencing of functional genes is a powerful technique to explore the diversity and abundance of microbes involved in biogeochemical processes. One such key process, denitrification, is of particular importance because it can transform nitrate (NO_3~-) to N_2 gas that is released to the atmosphere. In nitrogen limited alpine wetlands, assessing bacterial denitrification under the stress of wetland desertification is fundamental to understand nutrients, especially nitrogen cycling in alpine wetlands, and thus imperative for the maintenance of healthy alpine wetland ecosystems. We applied amplicon sequencing of the nirS gene to analyze the response of denitrifying bacterial community to alpine wetland desertification in Zoige, China. Raw reads were processed for quality, translated with frameshift correction, and a total of 95,316 nirS gene sequences were used for rarefaction analysis, and 1011 OTUs were detected and used in downstream analysis. Compared to the pristine swamp soil, edaphic parameters including water content, organic carbon, total nitrogen, total phosphorous, available nitrogen, available phosphorous and potential denitrification rate were significantly decreased in the moderately degraded meadow soil and in severely degraded sandy soil. Diversity of the soil nirS-type denitrifying bacteria communities increased along the Zoige wetland desertification, and Proteobacteria and Chloroflexi were the dominant denitrifying bacterial species. Genus Cupriavidus (formerly Wautersia), Azoarcus, Azospira, Thiothrix, and Rhizobiales were significantly (P<0.05) depleted along the wetland desertification succession. Soil available phosphorous was the key determinant of the composition of the nirS gene containing denitrifying bacterial communities. The proportion of depleted taxa increased along the desertification of the Zoige wetland, suggesting that wetland desertification created specific physicochemical conditions that decreased the microhabitats for bacterial denitrifiers and the denitrification related genetic diversity.
英文关键词Wetland desertification Amplicon sequencing nirS bacteria Differential abundance analysis
类型Article
语种英语
收录类别CSCD
WOS研究方向Agriculture
CSCD记录号CSCD:6492986
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/336113
作者单位Gu Yunfu, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.; Liu Ting, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.; Bai Yan, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.; Xiang Quanju, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.; Zhang Xiaoping, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.; Chen Qiang, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
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Gu Yunfu,Liu Ting,Bai Yan,et al. Pyrosequencing of nirS gene revealed spatial variation of denitrifying bacterial assemblages in response to wetland desertification at Tibet plateau[J],2019,16(5):1121-1134.
APA Gu Yunfu,Liu Ting,Bai Yan,Xiang Quanju,Zhang Xiaoping,&Chen Qiang.(2019).Pyrosequencing of nirS gene revealed spatial variation of denitrifying bacterial assemblages in response to wetland desertification at Tibet plateau.Journal of Mountain Science,16(5),1121-1134.
MLA Gu Yunfu,et al."Pyrosequencing of nirS gene revealed spatial variation of denitrifying bacterial assemblages in response to wetland desertification at Tibet plateau".Journal of Mountain Science 16.5(2019):1121-1134.
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