干旱区生态环境知识资源中心(Arid): Pyrosequencing of nirS gene revealed spatial variation of denitrifying bacterial assemblages in response to wetland desertification at Tibet plateau

Arid

	Pyrosequencing of nirS gene revealed spatial variation of denitrifying bacterial assemblages in response to wetland desertification at Tibet plateau
	Gu Yunfu; Liu Ting; Bai Yan; Xiang Quanju; Zhang Xiaoping; Chen Qiang
来源期刊	Journal of Mountain Science
ISSN	1672-6316
出版年	2019
卷号	16 期号:5 页码:1121-1134
英文摘要	Amplicon sequencing of functional genes is a powerful technique to explore the diversity and abundance of microbes involved in biogeochemical processes. One such key process, denitrification, is of particular importance because it can transform nitrate (NO_3~-) to N_2 gas that is released to the atmosphere. In nitrogen limited alpine wetlands, assessing bacterial denitrification under the stress of wetland desertification is fundamental to understand nutrients, especially nitrogen cycling in alpine wetlands, and thus imperative for the maintenance of healthy alpine wetland ecosystems. We applied amplicon sequencing of the nirS gene to analyze the response of denitrifying bacterial community to alpine wetland desertification in Zoige, China. Raw reads were processed for quality, translated with frameshift correction, and a total of 95,316 nirS gene sequences were used for rarefaction analysis, and 1011 OTUs were detected and used in downstream analysis. Compared to the pristine swamp soil, edaphic parameters including water content, organic carbon, total nitrogen, total phosphorous, available nitrogen, available phosphorous and potential denitrification rate were significantly decreased in the moderately degraded meadow soil and in severely degraded sandy soil. Diversity of the soil nirS-type denitrifying bacteria communities increased along the Zoige wetland desertification, and Proteobacteria and Chloroflexi were the dominant denitrifying bacterial species. Genus Cupriavidus (formerly Wautersia), Azoarcus, Azospira, Thiothrix, and Rhizobiales were significantly (P<0.05) depleted along the wetland desertification succession. Soil available phosphorous was the key determinant of the composition of the nirS gene containing denitrifying bacterial communities. The proportion of depleted taxa increased along the desertification of the Zoige wetland, suggesting that wetland desertification created specific physicochemical conditions that decreased the microhabitats for bacterial denitrifiers and the denitrification related genetic diversity.
英文关键词	Wetland desertification Amplicon sequencing nirS bacteria Differential abundance analysis
类型	Article
语种	英语
收录类别	CSCD
WOS研究方向	Agriculture
CSCD记录号	CSCD:6492986
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/336113
作者单位	Gu Yunfu, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.; Liu Ting, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.; Bai Yan, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.; Xiang Quanju, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.; Zhang Xiaoping, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.; Chen Qiang, Department of Microbiology, College of Resource Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
推荐引用方式 GB/T 7714	Gu Yunfu,Liu Ting,Bai Yan,et al. Pyrosequencing of nirS gene revealed spatial variation of denitrifying bacterial assemblages in response to wetland desertification at Tibet plateau[J],2019,16(5):1121-1134.
APA	Gu Yunfu,Liu Ting,Bai Yan,Xiang Quanju,Zhang Xiaoping,&Chen Qiang.(2019).Pyrosequencing of nirS gene revealed spatial variation of denitrifying bacterial assemblages in response to wetland desertification at Tibet plateau.Journal of Mountain Science,16(5),1121-1134.
MLA	Gu Yunfu,et al."Pyrosequencing of nirS gene revealed spatial variation of denitrifying bacterial assemblages in response to wetland desertification at Tibet plateau".Journal of Mountain Science 16.5(2019):1121-1134.