干旱区生态环境知识资源中心(Arid): Transcriptome analysis of the symbiosis-related genes between Funneliformis mosseae and Amorpha fruticosa

Arid

	Transcriptome analysis of the symbiosis-related genes between Funneliformis mosseae and Amorpha fruticosa
	Liu Xuan; Jiang Mingguo; Song Fuqiang
来源期刊	Journal of Forestry Research
ISSN	1007-662X
出版年	2019
卷号	30 期号:2 页码:483-495
英文摘要	Arbuscular mycorrhizal fungi (AMF) can colonize and form associations with the roots of Amorpha fruticosa L. (desert false indigo). Various genes are induced during the symbiotic process. In this study, de novo transcriptome sequencing using RNA-seq was conducted for the first time for a comprehensive analysis of AMF-A. fruticosa symbionts at the transcript level. We obtained 12G of raw data from illumina sequencing and recovered 115,786 unigenes with an average length of 547 bp, among them 41,848 of significance. A total of 2460 diffexpression genes were identified, including 1579 down-regulated and 881 up-regulated genes. A threshold for false discovery rate of <0.001 and fold change of >1 determined significant differences in gene expression. Using these criteria, we screened 285 significant differentially expressed genes, of which 82 were up-regulated and 203 down-regulated. The 82 up-regulated genes were classified according to their functions and assigned into seven categories: stress and defense, metabolism, signaling transduction, protein folding and degradation, energy, protein synthesis, and transcription. The 203 down-regulated genes were screened according to fold change >2, and 50 highly significant down-regulated genes were obtained related to stress and defense. The results of this study will provide a useful foundation for further investigation on the metabolic characteristics and molecular mechanisms of AMF associations with leguminous woody shrubs.
英文关键词	Amorpha fruticose Arbuscular mycorrhizal fungi RNA-seq Symbiosis-related gene Quantitative real-time PCR
类型	Article
语种	英语
收录类别	CSCD
WOS研究方向	Forestry
CSCD记录号	CSCD:6456001
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/336079
作者单位	Liu Xuan, Heilongjiang University, Harbin, Heilongjiang China.; Song Fuqiang, Heilongjiang University, Harbin, Heilongjiang China.; Jiang Mingguo, GuangXi University for Nationalities, Nanning, Guangxi China.
推荐引用方式 GB/T 7714	Liu Xuan,Jiang Mingguo,Song Fuqiang. Transcriptome analysis of the symbiosis-related genes between Funneliformis mosseae and Amorpha fruticosa[J],2019,30(2):483-495.
APA	Liu Xuan,Jiang Mingguo,&Song Fuqiang.(2019).Transcriptome analysis of the symbiosis-related genes between Funneliformis mosseae and Amorpha fruticosa.Journal of Forestry Research,30(2),483-495.
MLA	Liu Xuan,et al."Transcriptome analysis of the symbiosis-related genes between Funneliformis mosseae and Amorpha fruticosa".Journal of Forestry Research 30.2(2019):483-495.