干旱区生态环境知识资源中心(Arid): Endoplasmic reticulum stress transducer old astrocyte specifically induced substance contributes to astrogliosis after spinal cord injury

Arid

	Endoplasmic reticulum stress transducer old astrocyte specifically induced substance contributes to astrogliosis after spinal cord injury
	Takazawa Atsushi; Kamei Naosuke; Adachi Nobuo; Ochi Mitsuo
来源期刊	Neural Regeneration Research
ISSN	1673-5374
出版年	2018
卷号	13 期号:3 页码:536-540
英文摘要	Old astrocyte specifically induced substance (OASIS) is an endoplasmic reticulum (ER) stress transducer specifically expressed in astrocytes and osteoblasts. OASIS regulates the differentiation of neural precursor cells into astrocytes in the central nervous system. This study aimed to elucidate the involvement of ER stress responses stimulated via OASIS in astrogliosis following spinal cord injury. In a mouse model of spinal cord contusion injury, OASIS mRNA and protein expression were evaluated at days 7 and 14. A significant increase in OASIS mRNA on day 7 and an increase in protein on days 7 and 14 was observed in injured spinal cords. Immunostaining on day 7 revealed co-localization of OASIS and astrocytes in the periphery of the injury site. Furthermore, anti-OASIS small interfering RNA (siRNA) was injected at the injury sites on day 5 to elucidate the function of OASIS. Treatment with anti-OASIS siRNA caused a significant decrease in OASIS mRNA on day 7 and protein on days 7 and 14, and was associated with the inhibition of astrogliosis and hindlimb motor function recovery. Results of our study show that OASIS expression synchronizes with astrogliosis and is functionally associated with astrogliosis after spinal cord injury.
英文关键词	unfolded protein response cAMP-response element binding protein/activating transcription factor protein family C57BL/6 contusion injury reactive astrocyte functional recovery western blot real-time polymerase chain reaction immunohistochemistry glial fibrillary acidic protein
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	CSCD
WOS研究方向	Surgery
CSCD记录号	CSCD:6213392
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/335931
作者单位	Takazawa Atsushi, Department of Orthopaedic Surgery, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima Japan.; Adachi Nobuo, Department of Orthopaedic Surgery, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima Japan.; Ochi Mitsuo, Department of Orthopaedic Surgery, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima Japan.; Kamei Naosuke, Department of Orthopaedic Surgery, Graduate School of Biomedical & Health Sciences, Hiroshima University;;Medical Center for Translational & Clinical Research, Hiroshima University Hospital, ;;, Hiroshima;;Hiroshima, Japan;;Japan ;;.
推荐引用方式 GB/T 7714	Takazawa Atsushi,Kamei Naosuke,Adachi Nobuo,et al. Endoplasmic reticulum stress transducer old astrocyte specifically induced substance contributes to astrogliosis after spinal cord injury[J],2018,13(3):536-540.
APA	Takazawa Atsushi,Kamei Naosuke,Adachi Nobuo,&Ochi Mitsuo.(2018).Endoplasmic reticulum stress transducer old astrocyte specifically induced substance contributes to astrogliosis after spinal cord injury.Neural Regeneration Research,13(3),536-540.
MLA	Takazawa Atsushi,et al."Endoplasmic reticulum stress transducer old astrocyte specifically induced substance contributes to astrogliosis after spinal cord injury".Neural Regeneration Research 13.3(2018):536-540.