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	Highly sensitive assay for the determination of therapeutic peptide desmopressin in human plasma by UPLC-MS/MS
	Gudlawar Shiva Kumar; Pilli Nageswara Rao; Siddiraju Sridhar; Dwivedi Jaya
来源期刊	Journal of Pharmaceutical Analysis
ISSN	2095-1779
出版年	2017
卷号	7 期号:3 页码:196-202
英文摘要	An analytical method based on ultra-performance liquid chromatography with positive ion electrospray ionization (ESI) coupled with tandem mass spectrometry (UPLC-MS/MS) was developed and validated for the determination of therapeutic peptide desmopressin in human plasma. A desmopressin stable labeled isotope (desmopressin d_8) was used as an internal standard. Analyte and the internal standard were extracted from 200 muL of human plasma via solid-phase extraction technique using Oasis WCX cartridges. The chromatographic separation was achieved on an Aquity UPLC HSS T3 column by using a gradient mixture of methanol and 1 mM ammonium formate buffer as the mobile phase. The calibration curve obtained was linear (r~2≥0.99) over the concentration range of 1.01-200 pg/mL. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. The results of the intra- and inter-day precision and accuracy studies were well within the acceptable limits. The proposed method was successfully applied to pharmacokinetic studies in humans.
英文关键词	Desmopressin Solid-phase extraction (SPE) Ultra performance liquid chromatographytandem mass spectrometry Method validation Pharmacokinetics
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	CSCD
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:6013569
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/335823
作者单位	Gudlawar Shiva Kumar, Department of Chemistry, Banasthali Vidyapeeth, Jaipur, Rajasthan 304022, India.; Dwivedi Jaya, Department of Chemistry, Banasthali Vidyapeeth, Jaipur, Rajasthan 304022, India.; Pilli Nageswara Rao, Wellquest Clinical Research Laboratories, Hyderabad, Andhra Pradesh 500013, India.; Siddiraju Sridhar, Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Secunderabad, 5000100, India.
推荐引用方式 GB/T 7714	Gudlawar Shiva Kumar,Pilli Nageswara Rao,Siddiraju Sridhar,et al. Highly sensitive assay for the determination of therapeutic peptide desmopressin in human plasma by UPLC-MS/MS[J],2017,7(3):196-202.
APA	Gudlawar Shiva Kumar,Pilli Nageswara Rao,Siddiraju Sridhar,&Dwivedi Jaya.(2017).Highly sensitive assay for the determination of therapeutic peptide desmopressin in human plasma by UPLC-MS/MS.Journal of Pharmaceutical Analysis,7(3),196-202.
MLA	Gudlawar Shiva Kumar,et al."Highly sensitive assay for the determination of therapeutic peptide desmopressin in human plasma by UPLC-MS/MS".Journal of Pharmaceutical Analysis 7.3(2017):196-202.