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	Fast and sensitive LC-MS/MS method for the simultaneous determination of lisinopril and hydrochlorothiazide in human plasma
	Shah Jaivik V; Shah Priyanka A; Shah Priya V; Sanyal Mallika; Shrivastav Pranav S
来源期刊	Journal of Pharmaceutical Analysis
ISSN	2095-1779
出版年	2017
卷号	7 期号:3 页码:163-169
英文摘要	A sensitive and rapid liquid chromatography-tandem mass spectrometry(LC-MS/MS) method has been developed for the simultaneous determination of lisinopril(LIS) and hydrochlorothiazide(HCTZ) in human plasma using their labeled internal standards(ISs). Sample pre-treatment involved solid phase extraction on Waters Oasis HLB cartridges using 100 muL of plasma, followed by liquid chromatography on Hypersil Gold C18(50 mm*3.0 mm, 5 mum) column. The analytes were eluted within 2.0 min using acetonitrile-5.0 mM ammonium formate, pH 4.5(85:15, v/v) as the mobile phase. The analytes and ISs were analyzed in the negative ionization mode and quantified using multiple reaction monitoring. The method showed excellent linearity over the concentration range of 0.50-250.0 ng/mL for both the analytes. The intra-batch and inter-batch precision(% CV) was ≤5.26% and their extraction recoveries were in the range of 96.6%-103.1%. Matrix effect evaluated in terms of IS-normalized matrix factors ranged from 0.97 to 1.03 for both the analytes. The validated method was successfully applied to determine the plasma concentration of the drugs using 10 mg lisinopril and 12.5 mg hydrochlorothiazide fixed dose formulation in 18 healthy Indian volunteers.
英文关键词	Lisinopril Hydrochlorothiazide LC-MS/MS Solid phase extraction Human plasma
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	CSCD
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:6013564
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/335822
作者单位	Shah Jaivik V, Department of Chemistry, School of Sciences, Gujarat University, Ahmedabad, 380009, India.; Shah Priyanka A, Department of Chemistry, School of Sciences, Gujarat University, Ahmedabad, 380009, India.; Shrivastav Pranav S, Department of Chemistry, School of Sciences, Gujarat University, Ahmedabad, 380009, India.; Shah Priya V, Department of Pharmacy, M. S. University, Vadodara, 390002, India.; Sanyal Mallika, Department of Chemistry, St. Xavier's College, Ahmedabad, 380009, India.
推荐引用方式 GB/T 7714	Shah Jaivik V,Shah Priyanka A,Shah Priya V,et al. Fast and sensitive LC-MS/MS method for the simultaneous determination of lisinopril and hydrochlorothiazide in human plasma[J],2017,7(3):163-169.
APA	Shah Jaivik V,Shah Priyanka A,Shah Priya V,Sanyal Mallika,&Shrivastav Pranav S.(2017).Fast and sensitive LC-MS/MS method for the simultaneous determination of lisinopril and hydrochlorothiazide in human plasma.Journal of Pharmaceutical Analysis,7(3),163-169.
MLA	Shah Jaivik V,et al."Fast and sensitive LC-MS/MS method for the simultaneous determination of lisinopril and hydrochlorothiazide in human plasma".Journal of Pharmaceutical Analysis 7.3(2017):163-169.