干旱区生态环境知识资源中心(Arid): Buried straw layer and plastic mulching increase microflora diversity in salinized soil

Arid

	Buried straw layer and plastic mulching increase microflora diversity in salinized soil
	Li Yuyi; Pang Huancheng; Han Xiufang; Yan Shouwei; Zhao Yonggan; Wang Jing; Zhai Zhen; Zhang Jianli
来源期刊	Journal of Integrative Agriculture
ISSN	2095-3119
出版年	2016
卷号	15 期号:7 页码:1602-1611
英文摘要	Salt stress has been increasingly constraining crop productivity in arid lands of the world. In our recent study, salt stress was alleviated and crop productivity was improved remarkably by straw layer burial plus plastic film mulching in a saline soil. However, its impact on the microflora diversity is not well documented. Field micro-plot experiments were conducted from 2010 to 2011 using four tillage methods:(i) deep tillage with plastic film mulching(CK),(ii) straw layer burial at 40 cm(S),(iii) straw layer burial plus surface soil mulching with straw material(S+S), and(iv) plastic film mulching plus buried straw layer(P+S). Culturable microbes and predominant bacterial communities were studied; based on 16S rDNA, bacterial community structure and abundance were characterized using denaturing gradient gel electrophoresis(DGGE) and polymerase chain reaction(PCR). Results showed that P+S was the most favorable for culturable bacteria, actinomyces and fungi and induced the most diverse genera of bacteria compared to other tillage methods. Soil temperature had significant positive correlations with the number of bacteria, actinomyces and fungi(P<0.01). However, soil water was poorly correlated with any of the microbes. Salt content had a significant negative correlation with the number of microbers, especially for bacteria and fungi(P<0.01). DGGE analysis showed that the P+S exhibited the highest diversity of bacteria with 20 visible bands followed by S+S, S and CK. Moreover, P+S had the highest similarity(68%) of bacterial communities with CK. The major bacterial genera in all soil samples were Firmicutes, Proteobacteria and Actinobacteria. Given the considerable increase in microbial growth, the combined use of straw layer burial and plastic film mulching could be a practical option for alleviating salt stress effects on soil microbial community and thereby improving crop production in arid saline soils.
英文关键词	buried straw layer plastic mulch soil microflora changes PCR-DGGE microflora diversity salinized soil
类型	Article
语种	英语
开放获取类型	Other Gold
收录类别	CSCD
WOS研究方向	Agriculture
CSCD记录号	CSCD:5755924
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/335689
作者单位	Li Yuyi, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.; Pang Huancheng, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.; Zhao Yonggan, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.; Wang Jing, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.; Zhai Zhen, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.; Han Xiufang, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.; Yan Shouwei, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.; Zhang Jianli, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
推荐引用方式 GB/T 7714	Li Yuyi,Pang Huancheng,Han Xiufang,et al. Buried straw layer and plastic mulching increase microflora diversity in salinized soil[J],2016,15(7):1602-1611.
APA	Li Yuyi.,Pang Huancheng.,Han Xiufang.,Yan Shouwei.,Zhao Yonggan.,...&Zhang Jianli.(2016).Buried straw layer and plastic mulching increase microflora diversity in salinized soil.Journal of Integrative Agriculture,15(7),1602-1611.
MLA	Li Yuyi,et al."Buried straw layer and plastic mulching increase microflora diversity in salinized soil".Journal of Integrative Agriculture 15.7(2016):1602-1611.