干旱区生态环境知识资源中心(Arid): Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole (Cynoglossus semilaevis)

Arid

	Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole (Cynoglossus semilaevis)
	Dong Zhongdian; Qi Qian; Zhang Ning; Shao Changwei; Zhang Liyan; Wen Haishen; Chen Songlin
来源期刊	Acta Oceanologica Sinica
ISSN	0253-505X
出版年	2016
卷号	35 期号:6 页码:19-28
英文摘要	The signaling molecule hedgehog (Hh) is essential for cellular signaling required for body pattern formation in embryonic and individual development in vertebrate and invertebrates. Patched 1 (Ptc1), a receptor of Hh, mediates the activity of Hh by binging the Hh ligands. In this study, the Ptc1 of half-smooth tongue sole Cynoglossus semilaevis (CsPtc1) was cloned and characterized. The full-length of CsPtc1 cDNA is 5 212 nucleotides (nt), and encodes a protein with 1 543 amino acid residues. CsPtc1 shared many features with Ptc1 of other telesot species. Real-time quantitative PCR revealed CsPtc1 mRNA expressed in brain, liver, heart, gill, intestines, spleen, gonad and kidney, with expression level in testis significantly higher than in ovary. In testis, hybridization signals were mainly detected in primary spermatocytes, secondary spermatocytes, and sertoli cells, while weak signals were found in oocytes. Correspondingly, the degree of methylation is higher in female than in male and pseudomale. Results indicate that CsPtc1 may be involved in Desert Hedgehog (DHH) maintenance of the male and pseudo-male germ line and spermatogenesis.
英文关键词	half-smooth tongue sole male and female Patched 1 RACE qRT-PCR methylation
类型	Article
语种	英语
收录类别	CSCD
WOS研究方向	Fisheries
CSCD记录号	CSCD:5729883
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/335667
作者单位	Dong Zhongdian, Fisheries College, Ocean University of China, National Laboratory for Marine Science and Technology, Qingdao, Shandong 266003, China.; Qi Qian, Fisheries College, Ocean University of China, National Laboratory for Marine Science and Technology, Qingdao, Shandong 266003, China.; Zhang Ning, Fisheries College, Ocean University of China, National Laboratory for Marine Science and Technology, Qingdao, Shandong 266003, China.; Shao Changwei, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, National Laboratory for Marine Science and Technology, Qingdao, Shandong 266071, China.; Zhang Liyan, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, National Laboratory for Marine Science and Technology, Qingdao, Shandong 266071, China.; Chen Songlin, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, National Laboratory for Marine Science and Technology, Qingdao, Shandong 266071, China.; Wen Haishen, Fisheries College, Oce...
推荐引用方式 GB/T 7714	Dong Zhongdian,Qi Qian,Zhang Ning,et al. Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole (Cynoglossus semilaevis)[J],2016,35(6):19-28.
APA	Dong Zhongdian.,Qi Qian.,Zhang Ning.,Shao Changwei.,Zhang Liyan.,...&Chen Songlin.(2016).Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole (Cynoglossus semilaevis).Acta Oceanologica Sinica,35(6),19-28.
MLA	Dong Zhongdian,et al."Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole (Cynoglossus semilaevis)".Acta Oceanologica Sinica 35.6(2016):19-28.