干旱区生态环境知识资源中心(Arid): An HPLC-MS/MS method for the quantitative determination of platycodin D in rat plasma and its application to the pharmacokinetics of Platycodi Radix extract

Arid

	An HPLC-MS/MS method for the quantitative determination of platycodin D in rat plasma and its application to the pharmacokinetics of Platycodi Radix extract
	Zhan Qin; Zhang Feng; Gao Shouhong; Cai Fei; Jiang Bo; Sun Lianna; Chen Wansheng
来源期刊	Chinese Journal of Natural Medicines
ISSN	1672-3651
出版年	2014
卷号	12 期号:2 页码:154-160
英文摘要	AIMS: To develop an HPLC-MS/MS method for the quantification of platycodin D (PD) in rat plasma, and to acquire the main pharmacokinetic parameters of PD after oral administration of pure PD or of Platycodi Radix extract (PRE) containing PD. METHOD: Plasma samples were pretreated with solid-phase extraction using an Oasis? HLB SPE cartridge. Madecassoside was used as the internal standard (IS). Chromatographic separation was achieved on an ODS column (100 mm * 2.1 mm i.d., 3.5 mum) with a mobile phase consisting of acetonitrile/water (30 : 70, V/V) containing 0.1 mmol?L~(-1) ammonium acetate at a flow rate of 0.25 mL?min~(-1). The detection was performed on a triple quadruple tandem mass spectrometer using an elec-trospray ionization (ESI) source with a chromatographic run time of 3.0 min. The detection was operated by multiple reaction monitoring (MRM) of the transitions of m/z 1 223.6469.2 for PD and of m/z 973.6469.2 for madecassoside (IS), respec-tively. RESULTS: The calibration curve was linear from 5 to 2 000 ng?mL~(-1) (r~2 >0.99) with a lower limit of quantification (LLOQ) of 5 ng?mL~(-1). The intra- and inter-day precision (relative standard deviation, RSD) values were below 15% and the accuracy (relative error, RE) was from -15% to +15% at three quality control (QC) levels. Plasma concentrations of PD were deter-mined for 24 h after i.v. administration of PD, and oral administration of PD and PRE, respectively. The absolute oral bioa-vailability of PD in rats was found to be (0.48 0.19)% when administered PD, and to be (1.81 0.89)% when administered PRE. CONCLUSION: The developed HPLC-MS/MS method was successfully applied to assess the pharmacokinetic parameters and oral bioavailability of PD in rats after administration of PD and Platycodi Radix extract.
英文关键词	Platycodi Radix Platycodon grandiflorus Platycodin D HPLC-MS/MS Pharmacokinetics Rat plasma
类型	Article
语种	英语
收录类别	CSCD
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:5057629
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/335321
作者单位	Zhan Qin, Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.; Zhang Feng, Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.; Gao Shouhong, Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.; Cai Fei, Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.; Jiang Bo, Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.; Chen Wansheng, Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.; Sun Lianna, Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China.
推荐引用方式 GB/T 7714	Zhan Qin,Zhang Feng,Gao Shouhong,et al. An HPLC-MS/MS method for the quantitative determination of platycodin D in rat plasma and its application to the pharmacokinetics of Platycodi Radix extract[J],2014,12(2):154-160.
APA	Zhan Qin.,Zhang Feng.,Gao Shouhong.,Cai Fei.,Jiang Bo.,...&Chen Wansheng.(2014).An HPLC-MS/MS method for the quantitative determination of platycodin D in rat plasma and its application to the pharmacokinetics of Platycodi Radix extract.Chinese Journal of Natural Medicines,12(2),154-160.
MLA	Zhan Qin,et al."An HPLC-MS/MS method for the quantitative determination of platycodin D in rat plasma and its application to the pharmacokinetics of Platycodi Radix extract".Chinese Journal of Natural Medicines 12.2(2014):154-160.