干旱区生态环境知识资源中心(Arid): Microbial Activity and Culturable Bacterial Diversity in Sediments of the Great Rann of Kachchh, Western India

Arid

DOI	10.1016/S1002-0160(13)60079-X
	Microbial Activity and Culturable Bacterial Diversity in Sediments of the Great Rann of Kachchh, Western India
	Subrahmanyam G; Khonde N; Maurya D M; Chamyal L S; Archana G
来源期刊	Pedosphere
ISSN	1002-0160
出版年	2014
卷号	24 期号:1 页码:45-55
英文摘要	The Great Rann of Kachchh, a vast expanse of salt desert in Western India is a unique hostile ecosystem posing an extreme environment to life forms due to high salt content, hyper-arid climate, seasonal water logging and extremes of temperature. In the virtual absence of natural vegetation, soils and sediments of Rann of Kachchh are microbially dominated ecosystems. In the present study microbial activity and the diversity of cultivated heterotrophic bacteria were investigated in the sediments collected along a 5-m exposed section at Khadir Island in the Great Rann of Kachchh. Microbial activity (as an index of sediment enzymes) was found to be high in the middle of the section (200-280 cm). Dehydrogenase (DHA), substrate-induced DHA and alkaline phosphatase activities revealed the oligotrophic nature of the basal portion (320-480 cm). Abundant bacterial isolates obtained from different depths were found to be clustered in 12 different phylogenetic groups by amplified ribosomal DNA restriction analysis. 16S rRNA gene sequencing revealed the dominant bacterial ribotypes to be affiliated to Firmicutes (Families Bacillaceae and Staphylococcaceae) and Actinobacteria (Family Brevibacteriaceae) with minor contribution of Proteobacteria (Families Phyllobacteriaceae and Bartonellaceae), pointing their endurance and adaptability to environmental stresses. Statistical analysis indicated that sediment organic carbon, salinity, total available nitrogen and total available phosphorous are most likely critical determinants of microbial activity in the Khadir Island sediments.
英文关键词	amplified ribosomal DNA restriction analysis enzymes extreme environment heterotrophic bacteria 16S rRNA gene sequencing
类型	Article
语种	英语
收录类别	CSCD
WOS研究方向	Science & Technology - Other Topics
CSCD记录号	CSCD:5053893
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/335320
作者单位	Subrahmanyam G, Department of Microbiology and Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, 390002, India.; Archana G, Department of Microbiology and Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, 390002, India.; Khonde N, Department of Geology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, 390002, India.; Maurya D M, Department of Geology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, 390002, India.; Chamyal L S, Department of Geology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, 390002, India.
推荐引用方式 GB/T 7714	Subrahmanyam G,Khonde N,Maurya D M,et al. Microbial Activity and Culturable Bacterial Diversity in Sediments of the Great Rann of Kachchh, Western India[J],2014,24(1):45-55.
APA	Subrahmanyam G,Khonde N,Maurya D M,Chamyal L S,&Archana G.(2014).Microbial Activity and Culturable Bacterial Diversity in Sediments of the Great Rann of Kachchh, Western India.Pedosphere,24(1),45-55.
MLA	Subrahmanyam G,et al."Microbial Activity and Culturable Bacterial Diversity in Sediments of the Great Rann of Kachchh, Western India".Pedosphere 24.1(2014):45-55.