干旱区生态环境知识资源中心(Arid): Fungal diversity in adult date palm (Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches

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	Fungal diversity in adult date palm (Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches
	Ben Chobba Ines; Elleuch Amine; Ayadi Imen; Khannous Lamia; Namsi Ahmed; Cerqueira Frederique; Drira Noureddine; Gharsallah Neji; Vallaeys Tatiana
来源期刊	Journal of Zhejiang University. Science B, Biomedicine & Biotechnology
ISSN	1673-1581
出版年	2013
卷号	14 期号:12 页码:1084-1099
英文摘要	Endophytic flora plays a vital role in the colonization and survival of host plants, especial y in harsh en-vironments, such as arid regions. This flora may, however, contain pathogenic species responsible for various trou-blesome host diseases. The present study is aimed at investigating the diversity of both cultivable and non-cultivable endophytic fungal floras in the internal tissues (roots and leaves) of Tunisian date palm trees (Phoenix dactylifera). Accordingly, 13 isolates from both root and leaf samples, exhibiting distinct colony morphology, were selected from potato dextrose agar (PDA) medium and identified by a sequence match search wherein their 18S-28S internal transcribed spacer (ITS) sequences were compared to those available in public databases. These findings revealed that the cultivable root and leaf isolates fel into two groups, namely Nectriaceae and Pleosporaceae. Additional y, total DNA from palm roots and leaves was further extracted and ITS fragments were amplified. Restriction fragment length polymorphism (RFLP) analysis of the ITS from 200 fungal clones (leaves: 100; roots: 100) using HaeIII restriction enzyme revealed 13 distinct patterns that were further sequenced and led to the identification of Alternaria, Cladosporium, Davidiel a (Cladosporium teleomorph), Pythium, Curvularia, and uncharacterized fungal endophytes. Both approaches confirmed that while the roots were predominantly colonized by Fusaria (members of the Nectri-aceae family), the leaves were essential y colonized by Alternaria (members of the Pleosporaceae family). Overal, the findings of the present study constitute, to the authors knowledge, the first extensive report on the diversity of endophytic fungal flora associated with date palm trees (P. dactylifera).
英文关键词	Date palm tree (Phoenix dactylifera) Endophytic cultivable fungi rDNA internal transcribed spacer (ITS) Phylogenetic analysis Total DNA diversity analysis
类型	Article
语种	英语
开放获取类型	Green Published
收录类别	CSCD
WOS研究方向	Microbiology
CSCD记录号	CSCD:5020691
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/335298
作者单位	Ben Chobba Ines, Laboratoire de Biotechnologies Vegetales Appliquees a lAmelioration des Cultures LBVAAC, Faculte des Sciences de Sfax, Sfax, 3000, Tunisia.; Elleuch Amine, Laboratoire de Biotechnologies Vegetales Appliquees a lAmelioration des Cultures LBVAAC, Faculte des Sciences de Sfax, Sfax, 3000, Tunisia.; Drira Noureddine, Laboratoire de Biotechnologies Vegetales Appliquees a lAmelioration des Cultures LBVAAC, Faculte des Sciences de Sfax, Sfax, 3000, Tunisia.; Gharsallah Neji, Laboratoire de Biotechnologies Vegetales Appliquees a lAmelioration des Cultures LBVAAC, Faculte des Sciences de Sfax, Sfax, 3000, Tunisia.; Ayadi Imen, Universite de Sfax, Route Sidi Mansour, Sfax, 3018, Tunisia.; Khannous Lamia, Laboratoire de Phytopathologie Oasienne, Centre Regional de Recherches en Agriculture Oasienne a Degache, Degache, 2260, Tunisia.; Namsi Ahmed, Laboratoire de Phytopathologie Oasienne, Centre Regional de Recherches en Agriculture Oasienne a Degache, Degache, 2260, Tunisia.; Cerqueira Frederique, Pl...
推荐引用方式 GB/T 7714	Ben Chobba Ines,Elleuch Amine,Ayadi Imen,et al. Fungal diversity in adult date palm (Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches[J],2013,14(12):1084-1099.
APA	Ben Chobba Ines.,Elleuch Amine.,Ayadi Imen.,Khannous Lamia.,Namsi Ahmed.,...&Vallaeys Tatiana.(2013).Fungal diversity in adult date palm (Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches.Journal of Zhejiang University. Science B, Biomedicine & Biotechnology,14(12),1084-1099.
MLA	Ben Chobba Ines,et al."Fungal diversity in adult date palm (Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches".Journal of Zhejiang University. Science B, Biomedicine & Biotechnology 14.12(2013):1084-1099.