干旱区生态环境知识资源中心(Arid): Microbial Community Changes Along a Land-Use Gradient of Desert Soil Origin

Arid

DOI	10.1016/S1002-0160(12)60044-7
	Microbial Community Changes Along a Land-Use Gradient of Desert Soil Origin
	Wang Baozhan; Zhang Caixia; Liu Jiliang; Zeng Xiaowei; Li Fengrui; Wu Yucheng; Lin Xiangui; Xiong Zhengqin; Xu Jian; Jia Zhongjun
来源期刊	Pedosphere
ISSN	1002-0160
出版年	2012
卷号	22 期号:5 页码:593-603
英文摘要	Soil harbors remarkably stabilize bacterial communities at the phylum level. However, no two soils have exactly the same structure of bacterial phyla. The structure of microbial community is strongly influenced by the type of land-use through changes in soil attributes. Using high-throughput pyrosequencing and quantitative polymerase chain reaction techniques, soil microbial community structures were investigated along a land-use gradient of 100- and 27-year farmlands, a 33-year Pinus forest, a 28-year poplar forest, and a 21-year shrubland, as well as a native desert from which all cultivated systems were converted. The results revealed that the dominant phylotypes in the native soil comprised primarily of Alphaproteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes, accounting for > 71.4% of the total bacterial 16S rRNA sequence reads. Changes in land-use led to a significant decrease in these dominant phylotypes down to 33.4%. In contrast, the phylotypes with low abundance, such as Acidobacteria, Chloroflexi, Nitrospira, and Gammaproteobacteria, increased sharply from 4.5%-5.9% in the native soil to 20.9%-30.2% of the total 16S rRNA gene sequences in the cultivated soils except for the soil from the shrubland. These contrasting changes in the major taxa appear to be correlated with the changes in soil attributes. For instance, bacterial and archaeal amoA genes were found to be 960- and 3800-fold more abundant in the soil from the 100-year farmland than the native soil. The changes in numerically less dominant nitrifying phylotypes are consistent with soil inorganic nitrogen dynamics. Quantification of the 16S rRNA genes demonstrated that bacteria and archaea were about two to three orders of magnitude more abundant in the cultivated soil than in the native soil. Hence, land-use type affects the soil bacterial community structure, which has profound consequences on ecosystem function.
英文关键词	amoA ecosystem functions land-use type polymerase chain reaction pyrosequencing
类型	Article
语种	英语
收录类别	CSCD
WOS研究方向	Agriculture
CSCD记录号	CSCD:4650995
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/335165
作者单位	Wang Baozhan, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, Jiangsu 210008, China.; Zhang Caixia, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, Jiangsu 210008, China.; Wu Yucheng, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, Jiangsu 210008, China.; Lin Xiangui, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, Jiangsu 210008, China.; Jia Zhongjun, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, Jiangsu 210008, China.; Liu Jiliang, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China.; Li Fengrui, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China.; Zeng Xiaowei, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China.; Xu Jian, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy o...
推荐引用方式 GB/T 7714	Wang Baozhan,Zhang Caixia,Liu Jiliang,et al. Microbial Community Changes Along a Land-Use Gradient of Desert Soil Origin[J],2012,22(5):593-603.
APA	Wang Baozhan.,Zhang Caixia.,Liu Jiliang.,Zeng Xiaowei.,Li Fengrui.,...&Jia Zhongjun.(2012).Microbial Community Changes Along a Land-Use Gradient of Desert Soil Origin.Pedosphere,22(5),593-603.
MLA	Wang Baozhan,et al."Microbial Community Changes Along a Land-Use Gradient of Desert Soil Origin".Pedosphere 22.5(2012):593-603.