干旱区生态环境知识资源中心(Arid): Determination of Tetracyclines and Their Epimers in Agricultural Soil Fertilized with Swine Manure by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry

Arid

DOI	10.1016/S2095-3119(12)60114-2
	Determination of Tetracyclines and Their Epimers in Agricultural Soil Fertilized with Swine Manure by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry
	Zheng Wenli; Zhang Lifang; Zhang Keyu; Wang Xiaoyang; Xue Feiqun
来源期刊	Journal of Integrative Agriculture
ISSN	2095-3119
出版年	2012
卷号	11 期号:7 页码:1189-1198
英文摘要	A rapid, sensitive and specific ultra-high-performance liquid chromatography tandem mass spectrometry (UPLC-MS) method was developed for the analysis of tetracycline antibiotics, including tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC) and their 4-epimers (4-epiTCs) in agricultural soil fertilized with swine manure. Soil samples were extracted and cleaned-up with 10 mL EDTA-McIlvaine buffer solution (pH 4.0), then cleaned-up and pre-concentrated using the Oasis MAX cartridge and then eluted with 1 mL solution by mixing formic acid, methanol and water at a ratio of 2:15:83 (v/v/v). The purified samples were separated by an ACQUITY UPLC BEH C18 column using acetonitrile and water containing 0.1% formic acid mobile phase and detected by a single quadrupole MS. The limits of detection for the soil extraction method (LODsoil) ranged from 0.6-2.5 mug kg-1 with recoveries from 23.3-159.2%. Finally, the method was applied to an agricultural field in an area with intensive pig-fattening farming. Tetracyclines were detected in soil from 2.8 to 42.4 mug kg-1 soil. These results demonstrate that soil from swine farms can become severely contaminated with tetracycline antibiotics and their metabolites.
英文关键词	tetracyclines epimers ultra performance liquid chromatography soil solid phase extraction
类型	Article
语种	英语
开放获取类型	Other Gold
收录类别	CSCD
WOS研究方向	Agriculture
CSCD记录号	CSCD:4581545
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/335154
作者单位	Zheng Wenli, Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, 200241.; Zhang Lifang, Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, 200241.; Zhang Keyu, Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, 200241.; Wang Xiaoyang, Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, 200241.; Xue Feiqun, Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, 200241.
推荐引用方式 GB/T 7714	Zheng Wenli,Zhang Lifang,Zhang Keyu,et al. Determination of Tetracyclines and Their Epimers in Agricultural Soil Fertilized with Swine Manure by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry[J],2012,11(7):1189-1198.
APA	Zheng Wenli,Zhang Lifang,Zhang Keyu,Wang Xiaoyang,&Xue Feiqun.(2012).Determination of Tetracyclines and Their Epimers in Agricultural Soil Fertilized with Swine Manure by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry.Journal of Integrative Agriculture,11(7),1189-1198.
MLA	Zheng Wenli,et al."Determination of Tetracyclines and Their Epimers in Agricultural Soil Fertilized with Swine Manure by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry".Journal of Integrative Agriculture 11.7(2012):1189-1198.