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	A Study on Bisphenol A, Nonylphenol, and Octylphenol in Human Urine amples Detected by SPE-UPLC-MS
	Xiao Jing; Shao Bing; Wu Xiaoyan; Sun Xiaojie; Wu Yongning
来源期刊	Biomedical and Environmental Sciences
ISSN	0895-3988
出版年	2011
卷号	24 期号:1 页码:40-46
英文摘要	Objective To establish a comprehensive analytical method based on SPE-UPLC-MS for the simultaneous determination of bisphenol A (BPA), nonylphenol (NP), and octylphenol (OP) in urine samples. Methods Sixty urine samples collected from healthy subjects were analyzed for BPA, NP, and OP concentrations. The samples were de-conjugated by adding beta-glucuronidase and sulfatase. After the enzymatic treatment, the samples were subjected to the OASIS HLB column solid phase extraction cartridges so as to be cleaned and concentrated. The UPLC separation was performed on a Acquity UPLCTM BEH C18 column (2.1x100 mm, 1.7 mum) with a gradient elution system of methanol-water as the mobile phase. Triple-quadrupole mass spectrometry analyzer was used for the qualitative and quantitative analysis of UPLC-MS/MS system. Results The limit of detection of BPA, NP, and OP was 0.10, 0.10, and 0.15 ng/mL, respectively. The recoveries of BPA, NP and OP were 80.1%-108%, 81.3%-109%, and 81.5%-98.7%, respectively. Among the 60 urine samples, BPA was detected in 8 samples at the level of 0.297-32.7ng/mL, NP was detected in 29 samples at the level of 1.69-27.8 ng/mL, and OP was detected in 17 samples at the level of 0.407-11.1 ng/mL. Conclusion The method is simple with high sensitivity and selectivity, and is suitable for the determination of BPA, NP, and OP in urine. As shown by our analysis, BPA, NP, and OP appear to be prevalent in human urine. This is particularly true for NP. The results from our study is therefore valuable for future studies to assess the exposure to BPA, NP, and OP in the general population
英文关键词	Bisphenol A Nonylphenol Octylphenol Urine Solid phase extraction Ultra high performance liquid chromatography -mass/mass
类型	Article
语种	英语
收录类别	CSCD
WOS研究方向	Life Sciences & Biomedicine - Other Topics
CSCD记录号	CSCD:4148644
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/335085
作者单位	Xiao Jing, National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.; Wu Yongning, National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.; Shao Bing, Beijing Municipal Centrefor Disease Control and Prevention, Beijing 100013, China.; Wu Xiaoyan, Beijing Municipal Centrefor Disease Control and Prevention, Beijing 100013, China.; Sun Xiaojie, Beijing Municipal Centrefor Disease Control and Prevention, Beijing 100013, China.
推荐引用方式 GB/T 7714	Xiao Jing,Shao Bing,Wu Xiaoyan,et al. A Study on Bisphenol A, Nonylphenol, and Octylphenol in Human Urine amples Detected by SPE-UPLC-MS[J],2011,24(1):40-46.
APA	Xiao Jing,Shao Bing,Wu Xiaoyan,Sun Xiaojie,&Wu Yongning.(2011).A Study on Bisphenol A, Nonylphenol, and Octylphenol in Human Urine amples Detected by SPE-UPLC-MS.Biomedical and Environmental Sciences,24(1),40-46.
MLA	Xiao Jing,et al."A Study on Bisphenol A, Nonylphenol, and Octylphenol in Human Urine amples Detected by SPE-UPLC-MS".Biomedical and Environmental Sciences 24.1(2011):40-46.