干旱区生态环境知识资源中心(Arid): Influence of tonifying kidney recipe on advanced glycation endproducts and lipid peroxidation in ovariectomized rats

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	Influence of tonifying kidney recipe on advanced glycation endproducts and lipid peroxidation in ovariectomized rats
	Yuefen Wang; Changan Zhao; Li Guo; En Li
来源期刊	Neural Regeneration Research
ISSN	1673-5374
出版年	2008
卷号	3 期号:7 页码:737-741
英文摘要	BACKGROUND: Previous studies have demonstrated that reduced estrogen levels may accelerate the for-mation of advanced glycation endproducts (AGE) in brain tissue, raise the concentration of lipid peroxidation products in vivo, and speed up deterioration of learning and memory. A tonifying kidney recipe is hypothe-sized to improve the ability of learning and memory in ovariectomized rats by downregulating AGE and lipid peroxidation products.OBJECTIVE: To simulate a postmenopausal state, bilateral ovariectomy (OVX) was performed in rats, and the effects of tonifying kidney recipe (TKR) on AGE and lipid peroxidation in the rat cerebral cortex, hippo-campus, and blood serum levels was measured. In addition, the effects on learning and memory were evalu-ated, and the effect of AGE -specific inhibitor aminoguanidine (AG) was compared with TKR.DESIGN, TIME AND SETTING: A randomized, in vivo, control experiment was performed at the scien-tific research center (Provincial Key Laboratory) in the Fourth Hospital of Hebei Medical University (Shiji-azhuang, Hebei Province, China) from May 2005 to January 2007.MATERIALS: Forty healthy, adult, female, Sprague Dawley rats were used for this study. TKR was com-posed of prepared rehmannia rhizome, epimedium herb, desert-living cistanche, and Szechwan lovage rhi-zome, which were provided by Shijiazhuang Medical Materials Company (China). A TKR extraction was prepared for further use. AG was provided by Sigma (USA). Forty rats were randomly divided into four groups: sham, OVX, AG, and TKR, with 10 rats in each group.METHODS: The rat ovaries were resected in the OVX, AG, and TKR groups, whereas the same volume of fat was resected in the sham group. At four weeks after OVX, the AG group received 1% AG water solution by lavage; the TKR group was administrated by lavage once per day at a dose of 6.3 g (crude drug)/kg; OVX and sham groups received equal volumes of tap water.MAIN OUTCOME MEASURES: Learning and memory behavior of rats was tested in a Y-electric maze 16 weeks after the OVX procedure. The contents of advanced glycation endproducts in the rat cerebral cortex, hippocampus, serum, and urine were detected by competitive ELISA and spectrofluorophotometer. The con-tents of lipid peroxidation in rat serum, cerebral cortex, and hippocampus were assayed using a biochemical method.RESULTS: Compared with the sham group, serum content of advanced glycation endproducts in the OVX group was significantly increased, and lipid peroxidation content increased in cerebral cortex, hippocampus, and serum (t = 3.04-4.22, P < 0.05-0.01). Both AG and TKR decreased the amount of AGE in cerebral cor-tex and serum (t = 2.53, 3.64, P < 0.05, 0.01), increased AGE urine content (t = 3.25-4.87, P < 0.01), and decreased lipid peroxidation content in cerebral cortex, hippocampus, and serum (t = 2.80-3.70, P < 0.05-0.01). In comparison to the OVX and sham groups, the correct escape rate in the Y-electric maze was significantly increased (t = 3.46, 3.28, P < 0.01), and escape latency was significantly decreased (t=3.12, 2.48, P < 0.05) in the AG and TKR groups, which indicated that both AG and TKR improved learning and memory. The OVX group had a significantly lower correct escape compared with the sham group (t = 4.21, P < 0.01).CONCLUSION: The tonifying kidney recipe decreased deposition of advanced glycation endproducts and lipid peroxidation in ovariectomized rats, and concomitantly improved learning and memory. The effect of TKR was equal to that of AG.
英文关键词	advanced glycation endproducts aminoguanidine learning and memory lipid peroxidation ovariectomy tonifying kidney recipe
类型	Article
语种	英语
收录类别	CSCD
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:3347346
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334984
作者单位	Yuefen Wang, Department of Nephrology, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050017, China.; En Li, Department of Nephrology, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050017, China.; Changan Zhao, Department of Biochemistry and Molecular Biology, Xinxiang Medical College, Xinxiang, Hebei 453007, China.; Li Guo, Department of Biochemistry and Molecular Biology, Xinxiang Medical College, Xinxiang, Hebei 453007, China.
推荐引用方式 GB/T 7714	Yuefen Wang,Changan Zhao,Li Guo,et al. Influence of tonifying kidney recipe on advanced glycation endproducts and lipid peroxidation in ovariectomized rats[J],2008,3(7):737-741.
APA	Yuefen Wang,Changan Zhao,Li Guo,&En Li.(2008).Influence of tonifying kidney recipe on advanced glycation endproducts and lipid peroxidation in ovariectomized rats.Neural Regeneration Research,3(7),737-741.
MLA	Yuefen Wang,et al."Influence of tonifying kidney recipe on advanced glycation endproducts and lipid peroxidation in ovariectomized rats".Neural Regeneration Research 3.7(2008):737-741.