干旱区生态环境知识资源中心(Arid): Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification

Arid

	Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification
	Jiang Fan; Gu Yuanxin; Zheng Chaode; Fan Haifu
来源期刊	Chinese Physics
ISSN	1009-1963
出版年	2003
卷号	12 期号:8 页码:890-894
英文摘要	A new method of combining one-wavelength anomalous scattering (OAS) phasing and density modification has been described, in which the improved phases from density modification are re-introduced into OAS phasing. In this way, the phases could be improved iteratively until convergence. The OAS phasing method is based on the previously established sign-probability formula, which breaks the phase ambiguity in the OAS phasing. The implementation of this method has been available in CCP4 as OASIS. This method, although based on direct-methods, could also incorporate known phases and figures of merit into its sign-probability formula. In the implementation of OASIS, the known phases are from the positions of the anomalous scatters. In the current method, the known phases are from the density modification. The current method was tested on phasing a lysozyme crystal using anomalous scattering of sulphur atoms with diffraction data collected on an in-house x-ray source. The resulting map was well connected for the backbone atoms and clearly traceable, with an average map correlation coefficient of 0.6622 for the backbone atoms.
英文关键词	direct methods sign probability anomalous scattering density modification sulphur atom phasing
类型	Article
语种	英语
收录类别	CSCD
WOS研究方向	Physics
CSCD记录号	CSCD:1572936
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334852
作者单位	Jiang Fan, Institute of Physics, Chinese Academy of Sciences, BeiJing 100080, China.; Gu Yuanxin, Institute of Physics, Chinese Academy of Sciences, BeiJing 100080, China.; Zheng Chaode, Institute of Physics, Chinese Academy of Sciences, BeiJing 100080, China.; Fan Haifu, Institute of Physics, Chinese Academy of Sciences, BeiJing 100080, China.
推荐引用方式 GB/T 7714	Jiang Fan,Gu Yuanxin,Zheng Chaode,et al. Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification[J],2003,12(8):890-894.
APA	Jiang Fan,Gu Yuanxin,Zheng Chaode,&Fan Haifu.(2003).Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification.Chinese Physics,12(8),890-894.
MLA	Jiang Fan,et al."Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification".Chinese Physics 12.8(2003):890-894.