干旱区生态环境知识资源中心(Arid): Using autochthonous Bdellovibrio as a predatory bacterium for reduction of Gram-negative pathogenic bacteria in urban wastewater and reuse it

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	Using autochthonous Bdellovibrio as a predatory bacterium for reduction of Gram-negative pathogenic bacteria in urban wastewater and reuse it
	Jafarian, Neda; Sepahi, Abbas Akhavan; Naghavi, Nafiseh Sadat; Hosseini, Farzaneh; Nowroozi, Jamileh
通讯作者	Sepahi, AA (corresponding author), Islamic Azad Univ, Dept Microbiol, North Tehran Branch, Tehran, Iran.
来源期刊	IRANIAN JOURNAL OF MICROBIOLOGY
ISSN	2008-3289
EISSN	2008-4447
出版年	2020
卷号	12 期号:6 页码:556-564
英文摘要	Background and Objectives: The microbial contamination of wastewater is associated with health risks. The aim of this study was to use the autochthonous Bdellovibrio potential to prey Gram-negative pathogenic bacteria as a bio-control agent to treat urban wastewater. Materials and Methods: Thirty-six raw sewage samples were collected for isolation of Bdellovibrio. Double layer plaque assay was used for isolation and the isolates were identified by microscopic examination and molecular analysis. To evaluate the predatory potential for decrease number of Gram-negative pathogenic bacteria, plaque perdition assay, reduction in host cells viability by colony-forming unit (CFU) counting, reduction in optical density (OD) in co-cultures and assay of killing efficiency were carried out. Also, the raw wastewater was treated by Bdellovibrio then the reduction in CFU counting and reduction in OD was evaluated. Results: Four strains of Bdellovibrio were isolated and were registered in Gene Bank. Clear plaques were observed after 3-6 days of incubation for all prey cells. The CFU enumerations of all preys were decreased after 48 hrs in co-cultures and raw wastewater. Also, OD was decreased down to 0.2 nm after 48 hrs. Conclusion: These autochthonous Bdellovibrio strains are proposed to use for bio-control of Gram-negative pathogenic bacteria in wastewater and reuse it for irrigation in arid regions.
英文关键词	Autochthonous Bdellovibrio Predatory bacterium Bio-control Gram-negative pathogenic bacteria Waste water treatment
类型	Article
语种	英语
开放获取类型	Green Published
收录类别	ESCI
WOS记录号	WOS:000599985500008
WOS关键词	ORGANISMS ; MUNICIPAL ; SYSTEMS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334810
作者单位	[Jafarian, Neda; Sepahi, Abbas Akhavan; Hosseini, Farzaneh; Nowroozi, Jamileh] Islamic Azad Univ, Dept Microbiol, North Tehran Branch, Tehran, Iran; [Naghavi, Nafiseh Sadat] Islamic Azad Univ, Dept Microbiol, Falavarjan Branch, Esfahan, Iran
推荐引用方式 GB/T 7714	Jafarian, Neda,Sepahi, Abbas Akhavan,Naghavi, Nafiseh Sadat,et al. Using autochthonous Bdellovibrio as a predatory bacterium for reduction of Gram-negative pathogenic bacteria in urban wastewater and reuse it[J],2020,12(6):556-564.
APA	Jafarian, Neda,Sepahi, Abbas Akhavan,Naghavi, Nafiseh Sadat,Hosseini, Farzaneh,&Nowroozi, Jamileh.(2020).Using autochthonous Bdellovibrio as a predatory bacterium for reduction of Gram-negative pathogenic bacteria in urban wastewater and reuse it.IRANIAN JOURNAL OF MICROBIOLOGY,12(6),556-564.
MLA	Jafarian, Neda,et al."Using autochthonous Bdellovibrio as a predatory bacterium for reduction of Gram-negative pathogenic bacteria in urban wastewater and reuse it".IRANIAN JOURNAL OF MICROBIOLOGY 12.6(2020):556-564.