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DOI	10.14202/vetworld.2020.1884-1891
	Molecular diagnosis and biochemical studies of tick-borne diseases (anaplasmosis and babesiosis) in Aberdeen Angus Cattle in New Valley, Egypt
	Nasreldin, Nani; Ewida, Rania M.; Hamdon, Hatem; Elnaker, Yasser F.
通讯作者	Ewida, RM
来源期刊	VETERINARY WORLD
ISSN	0972-8988
EISSN	2231-0916
出版年	2020
卷号	13 期号:9 页码:1884-1891
英文摘要	Background and Aim: Anaplasmosis and babesiosis are tick-borne diseases that threaten livestock production with subsequent considerable economic losses. This study was conducted to diagnose Anaplasma and Babesia infection using molecular techniques in imported Aberdeen Angus cattle imported from Uruguay to El-Kharga Oasis in New Valley, Egypt, and to investigate the effects of disease on some serum biochemical and oxidative stress parameters. Materials and Methods: Blood samples were collected from 31 cattle, 21 diseased and ten apparently normal, of varying ages and sex. The blood was used for the preparation of blood smears, polymerase chain reaction assay, and separation of serum for biochemical investigation. The experimental production farm at the Faculty of Agriculture, New Valley University, was infested with ticks and variable clinical manifestations during the period from December 2017 to March 2018. One calf died of a suspected blood parasite infection. Results: The blood film examination revealed infection by blood parasites in 21 samples. Anaplasma marginale and Babesia bovis were identified in 12 and 14 samples, respectively. A total of 14 samples were examined by polymerase chain reaction (PCR) to make these identifications. Biochemical parameters showed significantly elevated serum alanine aminotransferase, aspartate aminotransferase, total bilirubin (T. Bil), and urea in blood from parasite-infected female cattle and male calves compared with controls. Increased serum total protein, globulin, and creatinine were recorded only in infected female cattle. The blood glucose level was significantly decreased in infected female cattle and male calves compared with controls. Furthermore, albumin and albumin/globulin ratio was significantly reduced in the infected female cattle. Oxidative stress profiles of infected animals showed a significant increase in serum nitric oxide and malondialdehyde, and both total antioxidant capacity and reduced glutathione (GSH) were significantly reduced in comparison with control animals. Conclusion: The incidence of A. marginale and B. bovis infection is high in imported Aberdeen Angus cattle in New Valley Province. PCR methods provide a short-term assessment of disease. An extensive epidemiological survey, employing serology together with molecular genetic methods, monitoring of abundance and distribution of tick vectors, availability of vaccination programs, and tracking of animal transport is also needed for control of blood parasites.
英文关键词	Aberdeen Angus cattle Anaplasma marginal Babesia bovis oxidative stress
类型	Article
语种	英语
开放获取类型	Green Published, Other Gold
收录类别	ESCI
WOS记录号	WOS:000580632600019
WOS关键词	BOVINE BABESIOSIS ; OXIDATIVE STRESS ; BLOOD-SAMPLES ; DOGS ; INFECTION ; SERUM ; BIGEMINA ; MARKERS ; PROFILE
WOS类目	Agriculture, Dairy & Animal Science ; Veterinary Sciences
WOS研究方向	Agriculture ; Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334684
作者单位	[Nasreldin, Nani] New Valley Univ, Dept Pathol & Clin Pathol, Fac Vet Med, POB 72511, El Kharga, Egypt; [Ewida, Rania M.] New Valley Univ, Dept Food Hyg Milk Hyg, Fac Vet Med, POB 72511, El Kharga, Egypt; [Hamdon, Hatem] New Valley Univ, Dept Anim Prod, Fac Agr, POB 72511, El Kharga, Egypt; [Elnaker, Yasser F.] New Valley Univ, Dept Anim Med Infect Dis, Fac Vet Med, POB 72511, El Kharga, Egypt
推荐引用方式 GB/T 7714	Nasreldin, Nani,Ewida, Rania M.,Hamdon, Hatem,et al. Molecular diagnosis and biochemical studies of tick-borne diseases (anaplasmosis and babesiosis) in Aberdeen Angus Cattle in New Valley, Egypt[J],2020,13(9):1884-1891.
APA	Nasreldin, Nani,Ewida, Rania M.,Hamdon, Hatem,&Elnaker, Yasser F..(2020).Molecular diagnosis and biochemical studies of tick-borne diseases (anaplasmosis and babesiosis) in Aberdeen Angus Cattle in New Valley, Egypt.VETERINARY WORLD,13(9),1884-1891.
MLA	Nasreldin, Nani,et al."Molecular diagnosis and biochemical studies of tick-borne diseases (anaplasmosis and babesiosis) in Aberdeen Angus Cattle in New Valley, Egypt".VETERINARY WORLD 13.9(2020):1884-1891.