干旱区生态环境知识资源中心(Arid): Biochemical characterization and thermodynamic study of lipase from psychrotolerant Pseudomonas punonensis

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DOI	10.1016/j.bcab.2020.101686
	Biochemical characterization and thermodynamic study of lipase from psychrotolerant Pseudomonas punonensis
	Sonkar, Kanchan; Singh, D. P.
通讯作者	Singh, DP
来源期刊	BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
EISSN	1878-8181
出版年	2020
卷号	28
英文摘要	A psychrotolerant lipolytic strain Pseudomonas punonensis was isolated from soil of cold desert of Leh (Laddakh) India. This strain was first time screened for production of extracellular lipase enzyme. The present study aimed for the production, partial purification, biochemical characterization, kinetic and thermodynamic parameters of lipase from this novel strain. Study of physico-chemical characteristic of lipase enzyme showed maximum activity at 30 degrees C and pH 9.0. Arrhenius plot of temperature dependent enzyme activity revealed the activation energy as 27.74 kJ/mol and the temperature quotient was calculated to be 1.01. The kinetic constants of the enzyme such as V max , and K m derived from Lineweaver- Burk plot of the enzyme activity recorded at 30 degrees C, were found to be 86.22 U mL(-1) and 0.163 mM respectively. Experiment on thermal inactivation of enzyme showed thermal inactivation energy (E-a(d)) to be 56.89 kJ/mol. Thermodynamic values of lipase were found as Gibb's free energy (87.44 kJ/mol), enthalpy (54.29 kJ/mol) and entropy (-0.109 kJ/mol/K) for the enzyme denaturation obtained at 30 degrees C. The thermodynamic values suggested that enzyme catalysis was spontaneous and efficient at 30 degrees C when compared with 50 and 70 degrees C.
英文关键词	Extracellular lipase Psychrotolerant Kinetics and thermodynamic property Thermal stability
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000576968500013
WOS关键词	ALKALINE LIPASE ; SUBMERGED FERMENTATION ; WASTE-WATER ; BACILLUS SP ; PURIFICATION ; IDENTIFICATION ; OIL ; HYDROLYSIS ; BACTERIUM
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334659
作者单位	[Sonkar, Kanchan; Singh, D. P.] Babasaheb Bhimrao Ambedkar Univ, Dept Environm Sci, Lucknow 226025, Uttar Pradesh, India
推荐引用方式 GB/T 7714	Sonkar, Kanchan,Singh, D. P.. Biochemical characterization and thermodynamic study of lipase from psychrotolerant Pseudomonas punonensis[J],2020,28.
APA	Sonkar, Kanchan,&Singh, D. P..(2020).Biochemical characterization and thermodynamic study of lipase from psychrotolerant Pseudomonas punonensis.BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY,28.
MLA	Sonkar, Kanchan,et al."Biochemical characterization and thermodynamic study of lipase from psychrotolerant Pseudomonas punonensis".BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY 28(2020).