干旱区生态环境知识资源中心(Arid): Optimization of PCR conditions for Agriophyllum, Haloxylon and Salsola microsatellite markers

Arid

DOI	10.26577/ijbch.2020.v13.i1.06
	Optimization of PCR conditions for Agriophyllum, Haloxylon and Salsola microsatellite markers
	Favarisova, N.; Kusmangazinov, A.; Karelova, D.; Yermagambetova, M.; Abugalieva, S., I
通讯作者	Abugalieva, SI
来源期刊	INTERNATIONAL JOURNAL OF BIOLOGY AND CHEMISTRY
ISSN	2218-7979
EISSN	2409-370X
出版年	2020
卷号	13 期号:1 页码:57-68
英文摘要	Microsatellites or Simple Sequence Repeat (SSR) are among the most informative and popular types of molecular markers for assessment of genetic diversity, population structure, construction of genetic maps, phylogenetics, phylogeography, systematics, etc. Hence, for reliable inference of results, optimization of various conditions involved in polymerase chain reaction (PCR) is a pre-requisite. The usage of the essential PCR components in optimal concentrations, as well as PCR conditions, determines the success of amplification. The present study was carried out to optimize conditions of PCR for further assessment of the genetic diversity of eight species in genera Agriophyllum, Haloxylon, and Salsola that belong to the Chenopodiaceae Vent. family. The list of species includes Agriophyllum squarrosum, Agriophyllum minus, Haloxylon aphyllum, Haloxylon persicum, Haloxylon ammodendron, Salsola arbuscula, Salsola arbusculiformis, and Salsola chiwensis. These selected species are widespread in desert regions of Kazakhstan and have great importance for sand dunes fixing, animal feeding, and other purposes.
英文关键词	Haloxylon Agriophyllum Salsola polymerase chain reaction microsatellite markers
类型	Article
语种	英语
开放获取类型	Other Gold
收录类别	ESCI
WOS记录号	WOS:000560915400006
WOS关键词	SSR MARKERS ; CROSS-AMPLIFICATION ; NUCLEAR ; POPULATIONS ; KAZAKSTAN ; TRANSFERABILITY ; CHENOPODIACEAE ; WHEAT ; LOCI
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334548
作者单位	[Favarisova, N.; Kusmangazinov, A.; Karelova, D.; Yermagambetova, M.; Abugalieva, S., I] Inst Plant Biol & Biotechnol, Alma Ata, Kazakhstan
推荐引用方式 GB/T 7714	Favarisova, N.,Kusmangazinov, A.,Karelova, D.,et al. Optimization of PCR conditions for Agriophyllum, Haloxylon and Salsola microsatellite markers[J],2020,13(1):57-68.
APA	Favarisova, N.,Kusmangazinov, A.,Karelova, D.,Yermagambetova, M.,&Abugalieva, S., I.(2020).Optimization of PCR conditions for Agriophyllum, Haloxylon and Salsola microsatellite markers.INTERNATIONAL JOURNAL OF BIOLOGY AND CHEMISTRY,13(1),57-68.
MLA	Favarisova, N.,et al."Optimization of PCR conditions for Agriophyllum, Haloxylon and Salsola microsatellite markers".INTERNATIONAL JOURNAL OF BIOLOGY AND CHEMISTRY 13.1(2020):57-68.