干旱区生态环境知识资源中心(Arid): De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing

Arid

DOI	10.1007/s13237-020-00324-1
	De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing
	Shivhare, Radha; Lakhwani, Deepika; Asif, Mehar H.; Chauhan, Puneet S.; Lata, Charu
通讯作者	Lata, C
来源期刊	NUCLEUS-INDIA
ISSN	0029-568X
EISSN	0976-7975
出版年	2020
卷号	63 期号:3 页码:341-352
英文摘要	Pearl millet (Pennisetum glaucumL.) is one of the most important small-grained annual cereal crops grown in the arid and semi-arid regions of India and Africa, where terminal drought is a major constraint for its productivity. Terminal drought stress is more damaging to pearl millet than drought at vegetative stage as 50% or more reduction in yield may incur. However, a pearl millet genotype, PRLT2/89-33, parent of a mapping population is tolerant to terminal drought stress. With the aim to understand the molecular mechanism underlying terminal drought tolerance in this naturally drought tolerant crop, we examined the leaf transcriptome of contrasting pearl millet genotypes namely, PRLT2/89-33 and H77/833-2 differing for terminal drought tolerance using illumina Miseq sequencing platform. In this study, 40,880 genes were found to be differentially expressed and 8273 unigenes showed significant similarities to known sequences in NCBI non-redundant (nr) database and were classified into 189 Gene Ontology and 24 Clusters of Orthologous Group terms for both the genotypes. Expression profiling of 13 randomly selected transcripts was done to validate the RNA-Seq data. The study revealed that the genes for phytohormones biosynthesis, secondary metabolites and abiotic stress related transcription factors were more expressed in PRLT2/89-33, throwing insights into the molecular basis of its terminal drought tolerance. The study also supported that Illumina Miseq Platform is a powerful tool for transcriptome analysis and molecular-marker development in economically important non-model crop species, particularly those with large and complex genomes.
英文关键词	Comparative transcriptome De novo assembly Pearl millet Terminal drought
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000541397400001
WOS关键词	TOLERANCE ; GENE ; IDENTIFICATION ; MECHANISMS ; YIELD ; TOOL
WOS类目	Cell Biology
WOS研究方向	Cell Biology
Scopus学科分类	Acad Sci & Innovat Res AcSIR, Ghaziabad 201002, India. ; Lata, C
CSCD记录号	CSCD:Natl Inst Sci Commun & Informat Resources, CSIR, 14 Satsang Vihar Marg, New Delhi 110067, India.
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334415
作者单位	[Shivhare, Radha; Lakhwani, Deepika; Asif, Mehar H.; Chauhan, Puneet S.; Lata, Charu] Natl Bot Res Inst, CSIR, Rana Pratap Marg, Lucknow 226001, Uttar Pradesh, India; [Shivhare, Radha; Asif, Mehar H.; Chauhan, Puneet S.; Lata, Charu] Acad Sci & Innovat Res AcSIR, Ghaziabad 201002, India; [Lata, Charu] Natl Inst Sci Commun & Informat Resources, CSIR, 14 Satsang Vihar Marg, New Delhi 110067, India
推荐引用方式 GB/T 7714	Shivhare, Radha,Lakhwani, Deepika,Asif, Mehar H.,et al. De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing[J],2020,63(3):341-352.
APA	Shivhare, Radha,Lakhwani, Deepika,Asif, Mehar H.,Chauhan, Puneet S.,&Lata, Charu.(2020).De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing.NUCLEUS-INDIA,63(3),341-352.
MLA	Shivhare, Radha,et al."De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing".NUCLEUS-INDIA 63.3(2020):341-352.