干旱区生态环境知识资源中心(Arid): Genetic diversity analysis of Rhanterium eppaposum Oliv. by ISSRs reveals a weak population structure

Arid

DOI	10.1016/j.cpb.2020.100138
	Genetic diversity analysis of Rhanterium eppaposum Oliv. by ISSRs reveals a weak population structure
	Al Salameen, Fadila; Habibi, Nazima; Al Amad, Sami; Kumar, Vinod; Dashti, Jamal; Talebi, Lina; Al Doaij, Bashayer
通讯作者	Al Salameen, F ; Habibi, N
来源期刊	CURRENT PLANT BIOLOGY
EISSN	2214-6628
出版年	2020
卷号	21
英文摘要	Rhanterium eppaposum Oliv. (Arfaj) the national plant of Kuwait, is a perennial bushy shrub and the national flower of Kuwait. It is one of the most palatable forage plants and an excellent performer for desert rehabilitation. Due to several anthropogenic activities and climate change, this native plant has suffered severe degradation. In order to formulate conservation strategies, the genetic diversity of Rhanterium, was assessed by inter simple sequence repeat (ISSR) markers. A total of 93 wild landraces distributed in six remote locations in Kuwait were tested using 17 primers (PIC = 0.31; polymorphism = 92.8 %) that produced 195 unambiguous and reproducible bands. Overall, diversity indices were high enough (Nei's gene diversity (h') - 0.286; Shannon's diversity index - 0.414) for these populations. AMOVA test returned a fixation coefficient (F-ST) of 0.116 (p = 0.0) with higher genetic variations detected within (88 %) the populations as compared to among (12 %) the populations. Satisfactory levels of genetic differentiation (G(ST) = 0.186; G'(ST) = 0.250; D' = 0.295) were recorded for R. eppaposum with a gene flow (N-M) maintained at 1.9. The maximum genetic distances were observed between Maqwa and Mina Abdulla (Ds- 0.158; F-ST- 0.228) whereas minimum was between Al Kabd and SANR (Ds - 0.06; F-ST- 0.05). A weak Mantel's correlation (r(2) - 0.139; P = 0.014) between the six populations was also recorded. A narrow population structure was observed through the PCoA (2 groups), STRUCTURE (2 groups, K = 2) and SPLIT decomposition (1 cluster). The high genetic diversity within the populations are encouraging, however the low genetic differentiation raises concerns. The population structure should be improved by planting genetically diverse genotypes in areas of very low diversity to broaden its genetic base.
英文关键词	Conservation management Genetic diversity Molecular markers Arfaj Polymorphism
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	ESCI
WOS记录号	WOS:000521128900004
WOS关键词	INBREEDING DEPRESSION ; EPAPPOSUM ; DISPERSAL ; POLLEN ; KUWAIT ; PLANTS ; FLOW
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334271
作者单位	[Al Salameen, Fadila; Habibi, Nazima; Al Amad, Sami; Kumar, Vinod; Al Doaij, Bashayer] Kuwait Inst Sci Res, Environm & Life Sci Res Ctr, Biotechnol Program, Shuwaikh, Kuwait; [Dashti, Jamal] Kuwait Inst Sci Res, Environm & Life Sci Res Ctr, Desert & Agr Ecosyst Program, Shuwaikh, Kuwait; [Talebi, Lina] Kuwait Inst Sci Res, Environm & Life Sci Res Ctr, Environm & Climate Change Program, Shuwaikh, Kuwait
推荐引用方式 GB/T 7714	Al Salameen, Fadila,Habibi, Nazima,Al Amad, Sami,et al. Genetic diversity analysis of Rhanterium eppaposum Oliv. by ISSRs reveals a weak population structure[J],2020,21.
APA	Al Salameen, Fadila.,Habibi, Nazima.,Al Amad, Sami.,Kumar, Vinod.,Dashti, Jamal.,...&Al Doaij, Bashayer.(2020).Genetic diversity analysis of Rhanterium eppaposum Oliv. by ISSRs reveals a weak population structure.CURRENT PLANT BIOLOGY,21.
MLA	Al Salameen, Fadila,et al."Genetic diversity analysis of Rhanterium eppaposum Oliv. by ISSRs reveals a weak population structure".CURRENT PLANT BIOLOGY 21(2020).