干旱区生态环境知识资源中心(Arid): Bacterial Communities of Novaya Zemlya Archipelago Ice and Permafrost

Arid

DOI	10.3390/geosciences10020067
	Bacterial Communities of Novaya Zemlya Archipelago Ice and Permafrost
	Below, Andrey A.; Cheptsov, Vladimir S.; Manucharova, Natalia A.; Ezhelev, Zakhar S.
通讯作者	Below, AA
来源期刊	GEOSCIENCES
EISSN	2076-3263
出版年	2020
卷号	10 期号:2
英文摘要	The study of bacterial communities associated with extreme ecosystems is one of the most important tasks in modern microbial ecology. Despite a large number of studies being performed, the ecosystems that have not been sufficiently explored from the microbiological point of view still exist. Such research is needed for improving the understanding of the limits and mechanisms of bacterial survival under extreme conditions, and for revealing previously undescribed species and their role in global biospheric processes and their functional specifics. The results of the complex microbiological characteristics of permafrost and ice-collected on the Severniy Island in the northern part of the Novaya Zemlya archipelago-which have not previously been described from microbiological point of view, are presented in this article. The analysis included both culture-independent and culture-dependent methods, in particular, the spectra of metabolic activity range analysis in vitro under different temperature, pH and salinity conditions. High values for the total number of prokaryotes in situ (1.9 x 10(8)-3.5 x 10(8) cells/g), a significant part of which was able to return to a metabolically active state after thawing, and moderate numbers of culturable bacteria (3.3 x 10(6)-7.8 x 10(7) CFU/g) were revealed. Representatives of Proteobacteria, Actinobacteria, and Bacteroidetes were dominant in situ; Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes were the most abundant phyla in vitro. Physiological assays revealed the mesophilic and neutrophilic optima of temperature and pH of culturing conditions, respectively, and wide temperature and pH ranges of culturable communities' reproduction activity. Isolated strains were characterized by moderate halotolerant properties and antibiotic resistance, including multiple antibiotic resistance. It was found that almost all cultured bacterial diversity revealed (not just a few resistant species) had extremotolerant properties regarding a number of stress factors. This indicates the high adaptive potential of the studied microbial communities and their high sustainability and capability to retain functional activity under changing environmental (including climatic) conditions in wide ranges.
英文关键词	culturable bacterial communities physiological profiling metabolic activity 16S rRNA sequencing stress tolerance antibiotic resistance
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	ESCI
WOS记录号	WOS:000519623600047
WOS关键词	ANCIENT SIBERIAN PERMAFROST ; SP NOV. ; MICROBIAL ECOLOGY ; LOW-TEMPERATURE ; ANTARCTIC PERMAFROST ; COLD DESERT ; ARCTIC ICE ; DIVERSITY ; LIFE ; SOIL
WOS类目	Geosciences, Multidisciplinary
WOS研究方向	Geology
Scopus学科分类	Network Researchers Chem Evolut Life CEL, Leeds 01924, W Yorkshire, England.
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334257
作者单位	[Below, Andrey A.; Cheptsov, Vladimir S.; Manucharova, Natalia A.; Ezhelev, Zakhar S.] Lomonosov Moscow State Univ, Soil Sci Fac, Moscow 119991, Russia; [Below, Andrey A.; Cheptsov, Vladimir S.] Network Researchers Chem Evolut Life CEL, Leeds 01924, W Yorkshire, England; [Cheptsov, Vladimir S.] Russian Acad Sci, Space Res Inst, Moscow 119991, Russia
推荐引用方式 GB/T 7714	Below, Andrey A.,Cheptsov, Vladimir S.,Manucharova, Natalia A.,et al. Bacterial Communities of Novaya Zemlya Archipelago Ice and Permafrost[J],2020,10(2).
APA	Below, Andrey A.,Cheptsov, Vladimir S.,Manucharova, Natalia A.,&Ezhelev, Zakhar S..(2020).Bacterial Communities of Novaya Zemlya Archipelago Ice and Permafrost.GEOSCIENCES,10(2).
MLA	Below, Andrey A.,et al."Bacterial Communities of Novaya Zemlya Archipelago Ice and Permafrost".GEOSCIENCES 10.2(2020).