干旱区生态环境知识资源中心(Arid): Isolation and Molecular identifications of Cellulase producing Bacteria from Desert Soil

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	Isolation and Molecular identifications of Cellulase producing Bacteria from Desert Soil
	Thamer, Albalawi H.; Pravej, Alam
通讯作者	Thamer, HA ; Pravej, A
来源期刊	RESEARCH JOURNAL OF BIOTECHNOLOGY
ISSN	2278-4535
出版年	2020
卷号	15 期号:3 页码:72-78
英文摘要	In this study, two isolates (PSAU1 and PSAU2) of aerobic bacteria were isolated from the desert soil (harsh environment temperature of 45 degrees C) of Saudi Arabia. The bacteria were isolated and their isolates (PSAU1 and PSAU2) were characterized through PCR amplification and a sequencing approach targeting the 16S RNA genes. Based on the sequencing results of the PSAU1 and PSAU2 isolates, 99% of the sequence identity were matched with the Bacillaceae family belonging to Bacillus megaterium (PSAU1) and Bacillus cereus (PSAU2) species. The cellulolytic activities of both isolates (PSAU1 and PSAU2) at 45 degrees C (pH 7.0) were also performed in the presence of carboxymethyl cellulose (CMC) used as a carbon source. The colonies isolated from PSAU1 and PSAU2 were grown on CMC agar medium and observed for 5 days to recover the maximum inhibition i.e. cellulase production using plate assay methods. The PSAU1 colony gave negative signals i.e. non-cellulolytic (B. megaterium) whereas the PSAU2 (B. cereus) isolate showed a positive signal or zone of inhibition on CMC-agar plates at 45 degrees C. Furthermore, cellulase activity was quantified in a CMC-supplemented medium and the maximum recovery of cellulases was 2.03 +/- 0.08 U/ml on 2 days of culture at 540 nm. Our observation indicates that desert soil is also a good source of cellulolytic microorganisms that can be useful for cellulose degradation in harsh environments (temperature > 40 degrees C). The fast growth of these isolates and cellulase activity are new contributions to the field of biotechnology, especially in the Arab region.
英文关键词	Cellulase Soil Bacteria PCR amplifications Activity
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000516755700012
WOS关键词	CELLULOLYTIC BACTERIA ; BACILLUS ; PURIFICATION ; ENZYMES
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334237
作者单位	[Thamer, Albalawi H.; Pravej, Alam] PSAU, Coll Sci & Humanities, Dept Biol, Alkharj 11942, Saudi Arabia
推荐引用方式 GB/T 7714	Thamer, Albalawi H.,Pravej, Alam. Isolation and Molecular identifications of Cellulase producing Bacteria from Desert Soil[J],2020,15(3):72-78.
APA	Thamer, Albalawi H.,&Pravej, Alam.(2020).Isolation and Molecular identifications of Cellulase producing Bacteria from Desert Soil.RESEARCH JOURNAL OF BIOTECHNOLOGY,15(3),72-78.
MLA	Thamer, Albalawi H.,et al."Isolation and Molecular identifications of Cellulase producing Bacteria from Desert Soil".RESEARCH JOURNAL OF BIOTECHNOLOGY 15.3(2020):72-78.