干旱区生态环境知识资源中心(Arid): Stimulation of 6-benzylaminopurine and meta-topolin-induced in vitro shoot organogenesis and production of flavonoids of Amburana cearensis (Allemao) A.C. Smith)

Arid

DOI	10.1016/j.bcab.2019.101408
	Stimulation of 6-benzylaminopurine and meta-topolin-induced in vitro shoot organogenesis and production of flavonoids of Amburana cearensis (Allemao) A.C. Smith)
	Costa Vasconcelos, Jessica Nascimento; Brito, Alone Lima; Pinheiro, Amanda Lima; Goncalves e Costa Pinto, Dinah Ise Jimenez; Guedes da Silva Almeida, Jackson Roberto; Soares, Taliane Leila; Ferreira de Santana, Jose Raniere
通讯作者	Soares, TL
来源期刊	BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
EISSN	1878-8181
出版年	2019
卷号	22
英文摘要	Amburana cearensis (cumaru) is a tree species native to the semi-arid region of northeastern Brazil. Extracts obtained from its bark, seeds and leaves are widely used in folk medicine. The growing demand for phytotherapic preparations from this species makes it necessary to develop techniques for commercial cultivation to supply inputs to the pharmaceutical industry. In this respect, in vitro culture is a viable alternative to meet this demand. The objective of this study was to evaluate the effect of two cytokinins, 6-benzylaminopurine (BAP) and metatopolin (Mtop), on the direct organogenesis and to establish the phytochemical profile of A. cearensis. In vitro multiplication was tested from leaf and cotyledon segments, inoculated in woody plant medium (WPM) supplemented with varying concentrations of BAP (0.00, 2.22, 4.44, 6.66 or 8.88 mu M) or Mtop (0.0, 2.0, 4.0, 6.0, 8.0 or 10.0 mu M). Phytochemical screening and high-performance liquid chromatography were carried out to establish the phytochemical profile of extracts from the aerial part of cumaru, obtained from plants resulting from conventional conditions in greenhouse, clonal shoots and from in vitro culture. The results allowed establishing a new protocol for in vitro regeneration of A. cearensis using Mtop. The extracts these plants grown in vitro contained a higher quantity of flavonoids than those grown under conventional conditions. The results obtained from this study provide valuable new information to support future works, so that the medicinal properties and the secondary metabolites produced by this plant can be further investigated in benefit to human health in sustainable form.
英文关键词	Cumaru In vitro culture Growth regulators BAP Mtop Secondary metabolites
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000509762700076
WOS关键词	PLANT-REGENERATION ; MICROPROPAGATION ; MULTIPLICATION ; COUMARIN ; FIDELITY ; WILLIAMS ; STRESS ; LEAVES
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334066
作者单位	[Costa Vasconcelos, Jessica Nascimento; Brito, Alone Lima; Pinheiro, Amanda Lima; Goncalves e Costa Pinto, Dinah Ise Jimenez; Soares, Taliane Leila; Ferreira de Santana, Jose Raniere] Univ Estadual Feira de Santana, Av Transnordestina S-N, BR-44036900 Feira De Santana, BA, Brazil; [Guedes da Silva Almeida, Jackson Roberto] Univ Fed Vale Sao Francisco, Av Jose de Sa Manicoba S-N, BR-56304917 Petrolina, PE, Brazil
推荐引用方式 GB/T 7714	Costa Vasconcelos, Jessica Nascimento,Brito, Alone Lima,Pinheiro, Amanda Lima,et al. Stimulation of 6-benzylaminopurine and meta-topolin-induced in vitro shoot organogenesis and production of flavonoids of Amburana cearensis (Allemao) A.C. Smith)[J],2019,22.
APA	Costa Vasconcelos, Jessica Nascimento.,Brito, Alone Lima.,Pinheiro, Amanda Lima.,Goncalves e Costa Pinto, Dinah Ise Jimenez.,Guedes da Silva Almeida, Jackson Roberto.,...&Ferreira de Santana, Jose Raniere.(2019).Stimulation of 6-benzylaminopurine and meta-topolin-induced in vitro shoot organogenesis and production of flavonoids of Amburana cearensis (Allemao) A.C. Smith).BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY,22.
MLA	Costa Vasconcelos, Jessica Nascimento,et al."Stimulation of 6-benzylaminopurine and meta-topolin-induced in vitro shoot organogenesis and production of flavonoids of Amburana cearensis (Allemao) A.C. Smith)".BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY 22(2019).