干旱区生态环境知识资源中心(Arid): CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM TILAPIA AND UTENSILS USED IN THE COMMERCIALIZATION OF TILAPIA IN THE STREET MARKETS OF A SEMI-ARID BRAZILIAN MUNICIPALITY

Arid

DOI	10.17306/J.AFS.2019.0727
	CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM TILAPIA AND UTENSILS USED IN THE COMMERCIALIZATION OF TILAPIA IN THE STREET MARKETS OF A SEMI-ARID BRAZILIAN MUNICIPALITY
	Correia, Tayla M. A.; Oliveira, Anay P. D.; Miyasato, Isabela F.; Santos, Tamires M. B.; Dias, Francesca S.
通讯作者	Dias, FS
来源期刊	ACTA SCIENTIARUM POLONORUM-TECHNOLOGIA ALIMENTARIA
ISSN	1644-0730
EISSN	1898-9594
出版年	2019
卷号	18 期号:4 页码:413-425
英文摘要	Background. The production and commercialization of tilapia (Oreochromis niloticus) is fundamentally important to the semi-arid region of northeastern Brazil. In this region, one of the main forms of commercialization occurs in street markets (fairs). A high incidence of methicillin-resistant Staphylococcus aureus (MRSA) strains was previously detected in different food-related environments in Brazil. However, limited data is available about the presence of MRSA in street markets commercializing tilapias. In this study, we identified Staphylococcus aureus from tilapia and utensils used in the commercialization of tilapia in the street markets of a semi-arid Brazilian municipality and characterized the virulence potential of the isolates by analyzing their beta-lactam resistance, intercellular adhesion and enterotoxin genes. Materials and methods. The study used samples from the 5 main markets in the city. Phenotypic tests to determine antimicrobial resistance, exopolysaccharide (EPS) production, the potential for biofilm formation and cell surface hydrophobicity were conducted on S. aureus isolates. The presence of antimicrobial resistance genes (mecA and blaZ), potential biofilm production genes (icaA and icaD) and enterotoxin (se) genes was investigated. Results. Coagulase-positive staphylococci (CoPS) were detected in samples from all markets in discordance with the legal limits in force. Twelve isolates were confirmed to be S. aureus. Ten isolates demonstrated multidrug resistance (MDR). All isolates were able to produce EPS and form biofilms. Eight isolates exhibited strong hydrophobicity and six a high potential for biofilm formation. Twelve isolates were positive for mecA, blaZ, icaD and sed. Conclusion. Tilapia marketed in unsuitable conditions may be a vehicle for staphylococcal food poisoning and for the dissemination of MRSA to consumers. Additionally, the ability of the isolates to produce biofilms is an alert to the presence and persistence of these virulent microorganisms on utensils used for the commercial distribution of tilapia.
英文关键词	tilapia MRSA antimicrobial resistance biofilm enterotoxins
类型	Article
语种	英语
开放获取类型	Bronze
收录类别	ESCI
WOS记录号	WOS:000507390300006
WOS关键词	METHICILLIN-RESISTANT ; BIOFILM FORMATION ; BOVINE MASTITIS ; SUSCEPTIBILITY ; MILK ; PCR
WOS类目	Food Science & Technology
WOS研究方向	Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/334053
作者单位	[Correia, Tayla M. A.; Oliveira, Anay P. D.; Miyasato, Isabela F.; Santos, Tamires M. B.; Dias, Francesca S.] Fed Univ San Francisco Valley UNIVASF, Rod BR 407,Km 12,Lote 543, BR-56300990 Petrolina, PE, Brazil
推荐引用方式 GB/T 7714	Correia, Tayla M. A.,Oliveira, Anay P. D.,Miyasato, Isabela F.,et al. CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM TILAPIA AND UTENSILS USED IN THE COMMERCIALIZATION OF TILAPIA IN THE STREET MARKETS OF A SEMI-ARID BRAZILIAN MUNICIPALITY[J],2019,18(4):413-425.
APA	Correia, Tayla M. A.,Oliveira, Anay P. D.,Miyasato, Isabela F.,Santos, Tamires M. B.,&Dias, Francesca S..(2019).CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM TILAPIA AND UTENSILS USED IN THE COMMERCIALIZATION OF TILAPIA IN THE STREET MARKETS OF A SEMI-ARID BRAZILIAN MUNICIPALITY.ACTA SCIENTIARUM POLONORUM-TECHNOLOGIA ALIMENTARIA,18(4),413-425.
MLA	Correia, Tayla M. A.,et al."CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM TILAPIA AND UTENSILS USED IN THE COMMERCIALIZATION OF TILAPIA IN THE STREET MARKETS OF A SEMI-ARID BRAZILIAN MUNICIPALITY".ACTA SCIENTIARUM POLONORUM-TECHNOLOGIA ALIMENTARIA 18.4(2019):413-425.